Extract of total glucosides of paeony and the preparing method thereof

[0038] Example 1: Preparation process of total paeoniflorin extract

[0039] Take 10kg of white peony decoction pieces, extract with 10L of 70% ethanol and reflux for 3 times, extract 1 hour each time, recover the solvent under reduced pressure to obtain the extract, add water to disperse and dissolve, so that the concentration of the aqueous solution is 2.84mg / mL (calculated by paeoniflorin), Centrifuge at 3000 rpm for 30 minutes, take the supernatant, pass 5L AB-8 macroporous adsorption resin, the adsorption flow rate is 1.0mL / min, the resin column diameter to height ratio is 1:8, and the sample amount is 7.68mg / mL (Based on the amount of paeoniflorin), 10% ethanol eluted 2 times the resin volume for impurity removal, the impurity removal flow rate was 1.0 mL / min, 70% ethanol eluted 5 times the resin volume, and the elution flow rate was 2.0 mL / min. Collect The 70% ethanol eluate, the solvent is recovered, and the vacuum is dried to obtain the total paeoniflorin extract. The tot...

[0040] Example 2: Preparation process of total paeoniflorin extract

[0041] Take 10kg of decoction pieces of white peony, 15L of 50% ethanol, reflux and extract 3 times, extract for 1.5 hours each time, recover the solvent, add water to disperse and dissolve the extract to make the concentration of the aqueous solution 3.00mg / mL (calculated by paeoniflorin), with 3000 revolutions Centrifuge for 30 min at a speed of 1 / min, take the supernatant, pass through 6L AB-8 macroporous adsorption resin, with an adsorption flow rate of 1.0 mL / min, a resin column diameter-to-height ratio of 1:7, and a sample amount of 7.00 mg / mL (with peony Glycoside), 10% ethanol eluted 2 times the resin volume for impurity removal, the impurity removal flow rate was 1.0 mL / min, 50% ethanol eluted 4 times the resin volume, the elution flow rate was 2.0 mL / min, and 50% ethanol was collected. After removing the liquid, recovering the solvent, and drying under reduced pressure, the total paeoniflorin extract i...

[0042] Example 3: Preparation process of total paeoniflorin extract

[0043] Take 10kg red peony decoction pieces, extract 2 hours of 70% ethanol and 12L reflux, extract the solvent for 2 hours each time, recover the solvent, add water to disperse and dissolve the extract, make the concentration of the aqueous solution 2.00mg / mL (calculated by the amount of paeoniflorin), at 3000 rpm / Centrifuge for 30 minutes at a rotation speed of 30 minutes, take the supernatant, and pass through 7L AB-8 macroporous adsorption resin, with an adsorption flow rate of 1.0 mL / min, a resin column diameter-to-height ratio of 1:9, and a sample amount of 8.00 mg / mL (total peony Glycoside meter), water eluted 4 times the volume of the resin for impurity removal, the impurity removal flow rate was 1.0 mL / min, 90% ethanol eluted 4 times the resin volume, the elution flow rate was 2.0 mL / min, collected 90% ethanol for elution Liquid, recover the solvent, and dry under reduced pressure to obtain the total p...