Using high-pressure homogenizing technology to process fresh plasma and fresh erythrocyie separated from animal blood

[0052] Preparation of plasma protein powder

[0053] Whole blood from healthy pigs was collected, anticoagulated with 4% trisodium citrate, and the anticoagulated whole blood was separated into heavy liquid and light liquid by using a tubular continuous separator. The heavy liquid was blood cell liquid, and the light liquid was plasma. The separation factor It is 4:6.

[0054] The plasma was homogenized with a high-pressure homogenizer (model GYB3022 purchased from Shanghai Donghua Homogenizer Factory). The applied pressure is shown in Table 1.

[0055] The plasma was sprayed dry with a high-speed centrifugal spray dryer (produced by Heilongjiang Dairy Machinery Factory), the air inlet temperature was 160° C., and the outlet air temperature was 68° C. to obtain off-white plasma protein powder.

[0056] Table 1

[0057] Example 1

[0059] Preparation of Hemoglobin Powder

[0060] Whole blood from healthy pigs was collected (purchased from the slaughterhouse of Shanghai Wufeng Shangshi Group), anticoagulated with 4% trisodium citrate, and separated into two parts, heavy liquid and light liquid, by a tubular continuous separator. The heavy liquid is blood cell fluid, the light liquid is plasma, and the separation factor is 4:6.

[0061] The plasma was homogenized with a high-pressure homogenizer (model GYB3022 purchased from Shanghai Donghua Homogenizer Factory). The applied pressure is shown in Table 2.

[0062] The plasma was sprayed dry with a high-speed centrifugal spray dryer (manufactured by Heilongjiang Dairy Machinery Factory), the air inlet temperature was 160° C., and the outlet air temperature was 68° C. to obtain dark brown hemoglobin powder.

[0063] Table 2

[0064] Example 8

[0066] Bactericidal efficacy test:

[0067] Mix a certain amount of Escherichia coli bacteria liquid into the fresh plasma, divide it into eight parts, seven of them, and process the feed liquid through a small high-pressure homogenizer (pressure 50MPa, 60MPa, 70MPa, 90MPa, 110MPa, 120MPa, 130MPa), and then Plasma protein powder (test group) was spray-dried by small spray drying equipment (inlet air temperature 160°C, outlet air temperature 68°C). The other group was directly spray-dried into plasma protein powder (control group) by small spray drying equipment (inlet air temperature 160°C, outlet air temperature 68°C).

[0068] Detection method: according to the national food microbiology detection standard (GB4789) to detect coliform bacteria.

[0069] The results are shown in Table 3.

[0070] table 3

[0071] group

coli index

Test group 50MPa

38

Test group 60MPa

26

Test group 70MPa

22

Test group 90MPa

16 ...