Method for determining citric acid concentration and citric acid determining reagent kit

[0035] The citric acid determination reagent of the present embodiment is a single reagent, comprising:

[0036] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0037] Stabilizer 500mmol / L

[0038] Reduced coenzyme 0.25mmol / L

[0039] Citrate lyase 10000U / L

[0040] Malate dehydrogenase 12000U / L

[0041] After the reagents are all dissolved and prepared, they are divided into bottles and freeze-dried to make dry powder reagents; before use, add purified water and reconstitute for use.

[0042] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, test secondary wavelength 405nm, the volume ratio of the measured citric acid sample to the reagent is 1 / 25 , the reaction direction is negative reaction (decreasing reaction), the delay time is about 0 minutes, and the detection time is about 5 minutes.

[0043] After adding the sample and reagent, let them mix and react...

[0045] The citric acid determination reagent of the present embodiment is double reagent, comprises:

[0046] Reagent 1

[0047] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0048] Stabilizer 50mmol / L

[0049] Reduced coenzyme 0.25mmol / L

[0050] Reagent 2

[0051] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0052] Stabilizer 500mmol / L

[0053] Citrate lyase 10000U / L

[0054] Malate dehydrogenase 12000U / L

[0055] After the reagents are all dissolved and prepared, they are divided into bottles to make liquid double reagents, which can be used directly.

[0056] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, test secondary wavelength 405nm, the volume ratio of the measured citric acid sample to reagent 1 and reagent 2 It is 2 / 20 / 5, the reaction direction is negative reaction (downward reaction), the delay time is about 0 minute...

[0059] The citric acid determination reagent of the present embodiment is three reagents, comprises:

[0060] Reagent 1

[0061] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0062] Stabilizer 50mmol / L

[0063] Reduced coenzyme 0.25mmol / L

[0064] Reagent 2

[0065] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0066] Stabilizer 500mmol / L

[0067] Malate dehydrogenase 12000U / L

[0068] Reagent 3

[0069] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0070] Stabilizer 500mmol / L

[0071] Citrate lyase 10000U / L

[0072] After all the reagents are dissolved and prepared, they are divided into bottles to make liquid three reagents, which can be used directly.

[0073] When measuring the concentration of citric acid, set it on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, test secondary wavelength 405nm, test citric ac...