Anti-hypertensive casein active peptide and method for producing the same as well as use thereof

[0026] 1. Enzyme reaction using enzyme reactor:

[0027] (1) Casein dissolution

[0028] Add 1000ml of water into the reactor, heat up to the optimum temperature of about 55°C, weigh 150g of casein (Shanghai Chemical Reagent Company), slowly and gradually put it into the water under magnetic stirring, adjust the pH to about 8.5 with 2mol / L NaOH, Under the condition of 55°C, all the casein was dissolved, so that the concentration of casein in the reactor was 15% (w / v, g / ml).

[0029] (2) Enzymolysis

[0030] According to the ratio of enzyme activity unit / substrate weight of 0.072AU / g casein, add 4.5g food-grade protease Alcalase (Novozymes Biotechnology Co., Ltd., 2.4L, enzyme activity 2.4AU / g), and maintain the reaction temperature at 55 ℃, and 2mol / L NaOH was added dropwise through the ZDJ-4A automatic potentiometric titrator to maintain the pH of the enzymolysis solution at about 8.5, and the enzymolysis time was 5 hours. The free amino nitrogen in the enzymolysis solutio...

[0038] Change the temperature in the above step 1(1) casein dissolution and step 1(2) enzymolysis to 60°C, pH to 8.6, substrate concentration to 12% (w / v, g / ml), and the rest to be implemented in the same way Example 1, preparing the antihypertensive peptide of the present invention.

[0040]Change the temperature during the above step 1(1) casein dissolution and step 1(2) enzymolysis to 58°C, the pH is 8.4, the substrate concentration is 18% (w / v, g / ml), and the rest are implemented in the same way Example 1, preparing the antihypertensive peptide of the present invention.