Medicine combination with antitumor activity

[0057] Example 1 Synergistic Combination of DMXAA and Pingyangmycin

[0058] Material:

[0059] Cell lines: human lung cancer A549 cells and liver cancer SMMC-7721 cells

[0060] Preparation of test drugs: DMXAA was dissolved with 5% sodium bicarbonate before use, and then made into the required concentration with RPMI1640 culture solution; Pingyangmycin (PYM) was dissolved with normal saline before use, and then made into the required concentration with RPMI1640 culture solution .

[0061] Cell culture:

[0062] Inoculate human lung cancer A549 cells and liver cancer SMMC-7721 cells into sterile culture flasks, add appropriate amount of RPMI1640 culture medium, and incubate at 37°C, 5% CO 2 cultured in an incubator with saturated humidity. The cells grow purely adherent and are passaged every 2-3 days. Digest with 3g / L trypsin for 1min during subculture, pipette with culture medium to make cell suspension, and inoculate according to the required concentration.

[0063...

[0086] Example 2 Synergistic combination of DMXAA and actinomycin D

[0087] Material:

[0088] Cell lines: human lung cancer A549 cells and liver cancer SMMC-7721 cells

[0089] Preparation of test drugs: DMXAA was dissolved with 5% sodium bicarbonate before use, and then made into the required concentration with RPMI1640 culture solution; actinomycin D was dissolved in normal saline before use, and then made into the required concentration with RPMI1640 culture solution.

[0090] Cell culture:

[0091] Inoculate human lung cancer A549 cells and liver cancer SMMC-7721 cells into sterile culture flasks, add appropriate amount of RPMI1640 culture medium, and incubate at 37°C, 5% CO 2 cultured in an incubator with saturated humidity. The cells grow purely adherent and are passaged every 2-3 days. Digest with 3g / L trypsin for 1min during subculture, pipette with culture medium to make cell suspension, and inoculate according to the required concentration.

[0092] method:...

[0115] Example 3 Synergistic combination of DMXAA and mitomycin (MMC)

[0116] Material:

[0117] Cell lines: human lung cancer A549 cells and liver cancer SMMC-7721 cells

[0118] Preparation of test drugs: DMXAA was dissolved with 5% sodium bicarbonate before use, and then made into the required concentration with RPMI1640 culture solution; mitomycin (MMC) was dissolved with normal saline before use, and then made into the required concentration with RPMI1640 culture solution. concentration.

[0119] Cell culture:

[0120] Inoculate human lung cancer A549 cells and liver cancer SMMC-7721 cells into sterile culture flasks, add appropriate amount of RPMI1640 culture medium, and incubate at 37°C, 5% CO 2 cultured in an incubator with saturated humidity. The cells grow purely adherent and are passaged every 2-3 days. Digest with 3g / L trypsin for 1min during subculture, pipette with culture medium to make cell suspension, and inoculate according to the required concentrat...