Saponin compound extracted from Momordica grosvenori root, preparation and use thereof

[0020] Get 3,000 grams of dried Luo Han Guo root fragments (30 mesh) collected in Longjiang Township, Yongfu, Guilin, Guangxi, soak them in 15 liters of methanol with a volume concentration of 80%, extract them under reflux at 80°C for 3 times, each time for 3 hours, filter, and combine the extractions liquid, and recover methanol under reduced pressure to obtain 120 grams of extract. The extract was dissolved in 2 liters of distilled water, filtered, and the filtrate was passed through a D101 type macroporous resin column, followed by water, methanol with a volume concentration of 50%, and methanol with a volume concentration of 100% for gradient elution, and the 100% methanol eluate was collected. Methanol was recovered under reduced pressure to obtain 5.1 g of extract, which was then chromatographed on a silica gel column and eluted with a gradient of a mixed solvent of chloroform:methanol:water=20:1:0.1~5:1:0.1 (v / v). Analysis and detection, collecting the fractions contai...

[0022] Get 2000 grams of dried Luo Han Guo root fragments (20 mesh) collected in Longjiang Township, Yongfu, Guilin, Guangxi, soak them in 8 liters of methanol with a volume concentration of 80%, reflux extraction at 80 ° C for 3 times, each time for 2 hours, filter, and combine the extractions Liquid, decompression recovery ethanol to obtain 78 grams of extract. The extract was dissolved in 1 liter of distilled water, filtered, and the filtrate was passed through an AB-8 type macroporous resin column, followed by water, methanol with a volume concentration of 50%, and methanol with a volume concentration of 100% for gradient elution, and collected for elution with 100% methanol liquid, methanol was recovered under reduced pressure to obtain 3.5 grams of extract, and then silica gel column chromatography was used for gradient elution with a mixed solvent of chloroform:methanol:water=20:1:0.1~5:1:0.1 (v / v). For layer chromatography detection, the fractions containing Siraitic a...

[0024] Get 3000 grams of dried Luo Han Guo root fragments (40 mesh) collected in Longjiang Township, Yongfu, Guilin, Guangxi, soak them in 18 liters of ethanol with a volume concentration of 90%, extract twice at 85° C. for 4 hours each time, filter, and combine the extracts , Ethanol was recovered under reduced pressure to obtain 130 grams of extract. The extract was dissolved in 2 liters of distilled water, filtered, and the filtrate was passed through a Diaion HP20 type macroporous resin column, followed by water, methanol with a volume concentration of 50%, and methanol with a volume concentration of 100% for gradient elution, and the 100% methanol eluate was collected , reclaim methanol under reduced pressure to obtain 5.7 grams of extract, then use silica gel column chromatography, elution with chloroform:methanol:water=20:1:0.1~5:1:0.1 (v / v) mixed solvent gradient, thin layer For chromatographic detection, the fractions containing Siraitic acid IIB and Siraitic acid IIC...