Novel method for preparing high-purity chlorogenic acid

[0051] The water extract of honeysuckle after deoiling with a pH value of 1 and a concentration of chlorogenic acid of 0.5g / L was used as a raw material, and a 4-stage multi-column series device was selected, and the filler was non-polar adsorption resin HPD-100A (Cangzhou Baoen Adsorption Material Science and Technology Co., Ltd.), the washing solution is hydrochloric acid aqueous solution with pH 3, the eluent is 10% ethanol-water solution, and the regeneration solution is absolute ethanol. Pump the raw material into the first-stage adsorption column at a flow rate of 1BV / h, switch to the second-stage adsorption column for adsorption at the same flow rate of the adsorption liquid after breakthrough, and press the flow rate of 5BV / h for the first-stage adsorption column after penetration. h, 1BV / h and 5BV / h are sequentially pumped into the washing liquid, eluent and regeneration liquid. After the second-stage column is adsorbed and penetrated, it is switched, and the impurity...

[0054] The water extract of Eucommia ulmoides after deoiling with a pH value of 4 and a chlorogenic acid concentration of 10 g / L was used as a raw material, a 64-stage multi-column series device was selected, and the filler was medium polarity adsorption resin AB-8 (Cangzhou Baoen Adsorption Material Technology Co., Ltd. Co., Ltd.), the washing liquid is hydrochloric acid aqueous solution of pH 3, the eluent is 50% ethanol, and the regeneration liquid is absolute ethanol. Pump the raw material into the first-stage adsorption column at a flow rate of 5BV / h, switch to the second-stage adsorption column for adsorption at the same flow rate of the adsorption liquid after breakthrough, and press the flow rate of 10BV / h for the first-stage adsorption column after penetration. h, 5BV / h and 10BV / h are sequentially pumped into the washing liquid, eluent and regeneration liquid. After the second-stage column is adsorbed and penetrated, it is switched, and the impurity washing, elution a...

[0057] The deoiled water extract of coffee beans with a pH value of 2 and a chlorogenic acid concentration of 1 g / L was used as raw material, a 32-stage multi-column series device was used, and the filler was polar adsorption resin HPD-600 (Cangzhou Baoen Adsorption Material Technology Co., Ltd. Co., Ltd.), the washing solution is hydrochloric acid aqueous solution with pH3, the eluent is 20% ethanol, and the regeneration solution is absolute ethanol. Pump the raw material into the first-stage adsorption column at a flow rate of 1BV / h, switch to the second-stage adsorption column for adsorption at the same flow rate of the adsorbent after breakthrough, and press the flow rate of 8BV / h for the first-stage adsorption column after penetration. h, 2BV / h and 8BV / h are sequentially pumped into the washing liquid, eluent and regeneration liquid. After the second-stage column is adsorbed and penetrated, it is switched, and the impurity washing, elution and regeneration are carried out...