Human Bcl-2 and human VEGF165 double-gene co-expression recombinant vector and building method thereof

[0042] Figure 1-11 Constitute the specific embodiment 1 of the present invention.

[0043] Human Bcl-2 and Human VEGF 165 Double-gene co-expression recombinant vector, including human Bcl-2 gene and human VEGF 165 Gene; wherein, the human Bcl-2 gene sequence is described in the sequence table SEQ ID No. 1; human VEGF 165 The gene sequence is as described in SEQ ID No. 2 in the sequence table; the vector is an adenovirus vector, and other vectors such as other adeno-associated viruses, lentiviruses and plasmids can also be used.

[0044] The human Bcl-2 and human VEGF 165 The construction process of the double gene co-expression recombinant adenoviral vector is as follows: figure 1 As shown, its construction method is as follows:

[0045] 1. Main experimental reagents and equipment:

[0046] Biochemical reagents: DNA Marker (Takara, Japan), tryptone, yeast extract, agar powder (Oxoid, Japan), agarose (Biowest, Spain), kanamycin, ampicillin (Amresco, USA) and Trizol reag...