Method for cultivating lactobacillus and producing live lactobacillus feed by utilizing scum

[0032] Embodiment 1. take starch sugar residue as the main raw material liquid batch culture of lactic acid bacteria

[0033] Bacteria: Lactobacillus rhamnosus.

[0034] (1) Preparation of seed solution

[0035] Seed medium: MRS medium

[0036] Strain preservation medium: MRS medium

[0037] Strain preservation method: 20% glycerol is stored at -40°C

[0038] Seed solution preparation method: put the preserved strains in an incubator at 37±5°C for static activation for 30 minutes, inoculate them into the seed medium at a ratio of 5% to 15%, and culture them statically at 37±5°C for 10-12 hours.

[0039] (2) Liquid culture

[0040] Liquid fermentation medium (g / L): sugar residue 100-200, peptone 7-15, MgSO 4 0.2~0.6, MnSO 4 0.03~0.1, after adjusting the pH, add appropriate amount of CaCO 3 To maintain a stable pH during fermentation.

[0041] Liquid fermentation conditions: inoculum size: 10%, culture temperature after inoculation: 37±5°C, static culture.

[0042] Fe...

[0052] Embodiment 2. take starch sugar residue as raw material for solid-state culture of lactic acid bacteria

[0053] Bacteria: Lactobacillus rhamnosus

[0054] (1) Preparation of seed liquid: with embodiment 1.

[0055] (2) Solid state culture

[0056] Solid-state fermentation medium: mainly sugar residue, with a small amount of bran added.

[0057] Solid-state fermentation conditions: inoculum size: 20%, cultivation temperature after inoculation: 37±5°C, humidity 40%-80%, fermentation time: 24h-36h.

[0058] Solid-state culture method: insert the seed solution into the fermentation medium with 20% inoculum amount, measure the OD value of the seed solution before inoculation, stir the material evenly and place it in an incubator at 37±5°C for 24h-36h to measure the number of viable bacteria .

[0059] (3) Detection of viable bacteria: the detection method of viable bacteria adopts the plate colony counting method.

[0060] The effect of raw material composition on the ...