Pangasianodon gigas lipid, application and method for preparing breast milk lipid substitutes thereof

[0036] 1. Extract fat from Mekong Delta catfish:

[0037] Wash the basa catfish and tracatfish in the Mekong Delta, remove the fish fillets, crush the remaining raw materials, and then conduct continuous cooking in a continuous cooking machine, press filter, remove residue and heat with steam After 20-60 minutes, the obtained crude fat is centrifuged, dehydrated through a flash evaporation process, and finally refined to obtain the finished product of Mekong Delta catfish fat.

[0038] The refining described in the examples of the present invention adopts mature technology, and the specific steps include: degumming, deacidification, decolorization and deodorization. The deodorization stage is carried out under the conditions of a temperature of 240° C., a residence time of 65 minutes, and a vacuum degree of 2.3 mbar.

[0039] 2. Oil fractionation:

[0040] Heat the Mekong Delta catfish fat obtained in the above steps to 55-60°C, stabilize for 20 minutes, then cool down to 30...

[0047] 1. Extraction of Mekong Delta catfish fat:

[0048] Wash the basa catfish and tracatfish in the Mekong Delta, cook them continuously, press filter, remove the slag, and heat them with steam for 20-60 minutes, then centrifuge the obtained crude fat, and then dehydrate through the flash evaporation process , and finally refined to obtain the Mekong Delta catfish fat product.

[0049] 2. Preparation of mixed fatty acids:

[0050] Mix canola oil, soybean oil and coconut oil in a weight ratio of 6:2:2, heat up to 100°C while stirring, keep for 20 minutes, and dehydrate. First add lye for saponification, separate glycerol, then add hydrochloric acid for acidification to obtain mixed fatty acids.

[0051] 3. Enzymatic transesterification:

[0052] The weight ratio of Mekong Delta catfish fat to mixed fatty acids is 1:1, the amount of lipase LIPOZYME RMIM added is 5% of the fat weight, the reaction temperature is 55° C., and the transesterification reaction time is 4 hours. ...

[0054] 1. Extract fat from Mekong Delta catfish:

[0055] See Example 1 for specific extraction steps.

[0056] 2. Oil fractionation:

[0057] See embodiment one for specific fractionation steps.

[0058] 3. Preparation of mixed fatty acid ethyl esters:

[0059] Mix corn oil, linseed oil and coconut oil in a weight ratio of 6:2:2, heat up to 100°C while stirring, keep for 20 minutes, and dehydrate. Add 1% catalyst KOH of oil weight into ethanol (the molar ratio of ethanol to oil is 6:1), and stir to dissolve. After the grease is cooled to 60°C, add the catalyst-added ethanol solution to start the reaction for 3 hours. Add citric acid solution (the molar ratio of citric acid to KOH is 1:1) and wash with water. Vacuum pump at 60°C for 1-2 hours. The final product is mixed fatty acid ethyl ester.

[0060] 4. Enzymatic transesterification:

[0061] The weight ratio of the fractionation liquid component of the Mekong Delta catfish to the mixed fatty acid ethyl ester obtaine...