Malaria vaccine compositions and constituents which elicit cell mediated immunity

[0043] Example 1, Selection of Variable Epitopes: Analysis of CSP Variable Regions

[0044] Variable regions 288 to 412 of the CSP protein from Plasmodium falciparum 3D7 ( image 3 The analysis of SEQ ID NO:15) was performed according to the IEDB database to determine whether the similarities and differences between the sequences were T cell antigenic determinants. Recognized T cell epitopes such as image 3 Shown in SEQ ID NO:16-21. The analysis results showed that the 22 amino acid sequences recognized in this variable region (residues 368-389 of SEQ ID NO: 15, in image 3 The CSP sequence in the box, numbered as SEQ ID NO: 38) contains an important part of the T cell epitope, which can be used to query the NCBI database to identify the peptide containing the 22 amino acids shown (KPKDELDYANDIEKKICKMEKC, SEQ ID NO: 38) Proteins with highly related amino acid sequences. These sequences with high identity were further analyzed by IEDB. The results are listed in Table 2....