Perfusion type bioartificial liver reactor based on double-layered nitrocellulose membrane
A nitrocellulose membrane and bioartificial technology, applied in the direction of suction equipment, etc., can solve the problems of difficulty in providing a three-dimensional hepatocyte culture microenvironment, lack of immune barrier and perfusion, uneven distribution of hepatocytes, etc., to avoid cell escape, Good cell compatibility, to achieve the effect of immune barrier
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Embodiment 1
[0022] Figure 1~3 The shown bioartificial liver reactor based on double-layer nitrocellulose membrane perfusion is an embodiment of the present invention, including a box body 1 and a cell scaffold 8 arranged in the inner cavity of the box body 1 . like figure 1 As shown, the cell support 8 adopts a double-layer nitrocellulose membrane, and the double-layer nitrocellulose membrane is arranged in the box body 1 in a wave-shaped folded shape. The left side and the right side of the box body 1 are defined as the two sides located in the direction of the long axis of the box body 1 . In the middle of the inner surface of the left side plate 2 of the casing 1 and the middle of the inner surface of the right side plate 3, grooves 9 equal in length to the two short sides of the double-layer nitrocellulose membrane are longitudinally arranged respectively, and the double-layer nitrocellulose membrane The two ends are fitted into the grooves 9 of the left side plate 2 and the right ...
Embodiment 2
[0025] like Figure 4 The shown bioartificial liver reactor based on double-layer nitrocellulose membrane perfusion is another embodiment of the present invention. The cell support 8 is folded in a W shape, and the top and bottom of the box 1 are aligned with the top of the bend. There is a space interval between them. The right side plate 3 of the box body 1 is a piston-type baffle plate that can perform linear reciprocating motion along the long axis direction in the box body. Adapt to different experimental needs.
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