Application of rhodamine B derivatives

[0024] 1) Add rhodamine B (2g) into a flask containing 20mL of ethylenediamine, stir the system electromagnetically, react at 80°C for 12 hours, remove unreacted solvent, separate and purify through a silica gel column to obtain rhodamine B-ethylenediamine Amine, its chemical structure formula is as follows:

[0025]

[0026] R=-NH 2 ,-N(CH 3 ) 2 ,-CH 2 -N(CH 3 ) 2

[0027] 2) Detection of the fluorescence emission intensity of rhodamine B-ethylenediamine (1 μg / mL) containing a 5-membered ring in the molecule in buffer solutions of different pH values ​​(7.0, 6.5, 6.0, 5.5, 5.0, 4.5, 4.0) . It was found that the fluorescence intensity of rhodamine B-ethylenediamine at pH4.0 was 800 times higher than that at pH7.0 (Ex: 530 / Em 560nm) (see figure 1 ), the acid of rhodamine B-ethylenediamine derivatives in embodiment 1 initiates and produces fluorescent chemical reaction scheme as follows:

[0028]

[0029]3) Rhodamine B-ethylenediamine (10 μg / mL) and lysosome probe...

[0034] Embodiment 2: Rhodamine B-N, the application of N-dimethylethylenediamine as cell lysosome fluorescent dye

[0035] 1) Add rhodamine B (2g) into a flask containing 20mL N,N-dimethylethylenediamine, stir the system electromagnetically, react at 80°C for 12h, remove unreacted solvent, separate and purify it through a silica gel column Rhodamine B-N,N-dimethylethylenediamine was obtained.

[0036] 2) Utilize rhodamine B-N, the step 2) of N-dimethylethylenediamine repeats embodiment 1), 3), 4), 5), 6), 7) the result obtained and rhodamine B-ethylenediamine derivative The experimental results of the two substances are similar, indicating that the performance of the two as specific probes for lysosomes in cells is basically the same.

[0037] Embodiment 3: the application of Rhodamine B-propylenediamine as the fluorescent dye of cell lysosome

[0038] 1) Add rhodamine B (2g) into a flask containing 20mL propylenediamine, stir the system electromagnetically, react at 80°C for 12h, remove unreacted solvent, separate and purify through a silica gel column to obtain rhodamine B-propanediamine diamine.

[0039] 2) utilize rhodamine B-propylenediamine to repeat the step 2) of embodiment 1), 3), 4), 5), 6), 7) the result obtained is similar to the experimental result of rhodamine B-ethylenediamine, illustrates The performances of the two as specific probes for lysosomes of cells are basically the same.