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Agar culture medium and preparation method thereof

An agar medium and nutrient solution technology, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve the problems of inconspicuous black hearts, blurring, missed detection, etc., and achieve the effect of easy observation and solving missed detection.

Inactive Publication Date: 2012-06-13
JIANGMEN KAILIN TRADE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology improves upon existing methods by allowing for better observation on specific types of microorganisms found within these media compared with other techniques such as culture or analysis. By doing this, it can help identify any issues related to missing testing during routine monitoring procedures without having to visit foreign locations where they may be harming their health. Colon sizes range from 4-5% upward depending on how well the negative bacillus strain works against certain substances like chloroform gas (CFC) used in clean rooms.

Problems solved by technology

This patented technical problem addressed by this patents relates to finding out whether certain microorganisms are present or absent from their colonies due to lacking specific areas called dark zones on them's surface.

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0018] A kind of agar medium, its composition and mass number are: in every 1000ml nutrient solution: peptone 3g, lactose 8g, beef extract powder 3g, No. 3 bile salt 7g, neutral red 0.027g, agar 17g, sodium citrate 9.5g g, ferric citrate 1.7g, brilliant green 0.00036g, sodium thiosulfate 8.8g.

Embodiment 2

[0020] A kind of agar medium, its composition and mass number are: in every 1000ml nutrient solution: peptone 7g, lactose 12g, beef extract powder 3g, No. 3 bile salt 3g, neutral red 0.023g, agar 1, sodium citrate 7.5g g, ferric citrate 1.3g, brilliant green 0.0003g, sodium thiosulfate 8.2g.

Embodiment 3

[0022] An agar medium, its composition and mass number are: in every 1000ml of nutrient solution: 3g-7g of peptone, 10g of lactose, 5g of beef extract powder, 5g of No. 3 bile salt, 0.05g of neutral red, 15g of agar, citric acid Sodium 8g, ferric citrate 15g, brilliant green 0.00033g, sodium thiosulfate 8.5g.

[0023] In the above three embodiments, the main components of the nutrient solution are amino acids, vitamins, and growth factors.

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Abstract

The invention discloses an agar culture medium. Each 1,000 milliliters of nutrient solution comprises the following components by mass: 3 to 7 grams of peptone, 8 to 12 grams of lactose, 3 to 7 grams of beef extract powder, 3 to 7 grams of No.3 cholate, 0.023 to 0.027 gram of neutral red, 13 to 17 grams of agar, 7.5 to 9.5 grams of sodium citrate, 1.3 to 1.7 grams of ferric citrate, 0.0003 to 0.00036 gram of brilliant green and 8.2 to 8.8 grams of sodium thiosulfate. The preparation method comprises the following steps of: 1) dissolving 5 grams of peptone, 10 grams of lactose, 5 grams of beef extract powder, 5 grams of No.3 cholate, 15 grams of agar, 8 grams of sodium citrate, 1.5 grams of ferric citrate and 8.5 grams of sodium thiosulfate in 1,000 milliliters of nutrient solution; 2) heating; 3) regulating the pH value to be between 7.0+/-0.1, and adding 0.025 gram of neutral red and 0.00033 gram of brilliant green; 4) sterilizing; and 5) refrigerating.

Description

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Claims

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Application Information

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Owner JIANGMEN KAILIN TRADE
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