Preparation method of dye nanoparticle with aqueous phase dispersion and adjustable wavelength

[0037] A method for preparing aqueous dispersion wavelength-tunable dye nanoparticles, which comprises the following steps:

[0038] Step 1) Prepare two hydrophobic organic fluorescent dye molecules into doped nanoparticles by solvent exchange method; the specific method is to mix the two organic fluorescent dye molecules according to the molar ratio of 20:0.05-20:4 Prepare a mixed fluorescent dye molecular solution in a good solvent, take an appropriate volume of the organic fluorescent dye molecular mixed solution and add it to an appropriate amount of poor solvent, stir magnetically for 3 minutes and then let stand for 6 hours to obtain doped fluorescent nanoparticles;

[0039] Step 2) Take the amphiphilic surfactant and configure it into a 1 mg / mL amphiphilic surfactant aqueous solution; add 200 μL of the 1 mg / mL amphiphilic surfactant aqueous solution to the suspension of the precursor nanoparticles, and ultrasonically After dispersing for 5 minutes and standing still for...

[0052] In this example, three kinds of hydrophobic organic fluorescent dye molecules were used to prepare doped nanoparticles, and the molar ratio of the three small organic dye molecules was 20:0.25:0.05-20:0.25:2. Other steps are with embodiment 1.

[0053] Preferably, the molar ratio of the three hydrophobic organic fluorescent dye molecules is 20:0.25:0.5

[0055] The present invention will be further described by taking the hydrophobic organic fluorescent dye molecule 2-tert-butyl-9,10-di(2-naphthyl)anthracene and coumarin system as an example:

[0056] Firstly, two small organic dye molecules were mixed and dissolved in tetrahydrofuran solvent with a molar ratio of 20:0.05.

[0057] Secondly, uniformly dispersed 2-tert-butyl-9,10-di(2-naphthyl)anthracene-coumarin-doped fluorescent nanoparticles were obtained by solvent exchange method, with an average kinetic diameter of 98nm.

[0058] Finally, 200 μL of 1 mg / mL amphiphilic surfactant was added to 60 μmol of 2-tert-butyl-9,10-bis(2-naphthyl)anthracene-coumarin-doped fluorescent nanoparticles solution, and ultrasonically dispersed for 5 minutes , standing still for 6 hours, prepared comfortable fluorescent nanoparticles with an average kinetic particle diameter of 110nm dispersed in the aqueous phase, which has high stability in physiological saline, and the kinetic diameter rem...