Mycobacterium tuberculosis rifampin-resistance mutation visual detection probes and application thereof

[0062] 1. Design of DNA peroxidase mutation detection probe system

[0063] (1) The sequence of the core region of the rpoB gene mutation is as follows: figure 1 shown

[0064] The present invention comprises 22 mutation types of 510 to 524 amino acids ( figure 1 underlined), the specific mutations and corresponding detection probes are as follows:

[0065] probe cluster

mutation site

mutation type

Corresponding detection probe

1

510

G→C

B510-3G

1

511

C→T

B511-1A

1

511

T→C

B511-2G

1

511

T→G

B511-2C

1

513

C→A

B513-1T

1

513

A→C

B513-2G

1

513

A→G

B513-2C

2

515

A→G

B515-1C

2

515

G→T

B515-3A

2

516

G→A

B516-1T

2

516

G→T

B516-1A

2

516

A→C

B516-2G

2

516

A→G

B516-2C

...