Visualized detection probes and application thereof in tuberculosis rifampicin resistance mutation detection

The invention discloses novel visualized fast and sensitive detection probes and applications thereof in mycobacterium tuberculosis rifampicin resistance mutation detections. The visualized probes provided by the invention comprise: a common anchoring probe A, insulation probes L and R, mutation detection probes B, and a common competition probe SIB. The mutation detection probes B and the probe SIB have a same target DNA complementary region, and only the base types of the mutation sites are different. Aims at rifampicin resistance mutation, a detection probe cluster is designed and synthesized according to all the mutation sites included in a mycobacterium tuberculosis rifampicin resistance rpoB genetic mutation core. The probe cluster comprises 8 probe sets, wherein each probe set comprises a specific mutation detection probe B. With the method provided by the invention, the designed probe sets and probe clusters have good distinguishing ability upon synthesized wild-type and mutant detection fragments, and can be used for accurately detecting specific mutation sites of 530-533 site amino acids of the mycobacterium tuberculosis rifampicin resistance rpoB gene.