Application of oil orange to preparation of anti-H1N1 flu medicament

[0019] (I) Preparation of flavonoids extract from Amla emblica

[0020] Take 2000g of emblica medicinal material, add 10 times the amount of water to extract 3 times, filter, collect the filtrate, concentrate to 2500ml, add hydrochloric acid to adjust the pH to 3-4, and filter; the filtrate is passed through D-101 macroporous adsorption resin column, first Wash with water until the pH of the effluent is neutral, then wash the column with ethanol with a volume concentration of 70%, collect the ethanol eluate and concentrate to contain 1g of emblica crude drug in every milliliter of medicinal liquid, and the concentrated solution uses n-butanol extracting, concentrating the extracting solution to obtain, and drying to obtain powdered emblica flavonoid extract.

[0021] (Ⅱ) Determination of total flavonoids in emblica flavonoids extract

[0022] 1. Instruments and reagents

[0023] 1.1 Instrument

[0024] SPECTRONIC GENESYSTM 2 UV-visible spectrophotometer (SPECTRONIC, USA), C...

[0054] (I) Preparation of flavonoids extract from Amla emblica

[0055] Take 1500g of emblica medicinal material, add 8 times the amount of water to extract 3 times, filter, collect the filtrate, concentrate to 1500ml, add hydrochloric acid to adjust the pH to 2-3, take the acidic filtrate to pass through the macroporous adsorption resin column, the type of macroporous resin used For D-101-I, first wash with water until the effluent is neutral; then add 70% ethanol to wash the column; collect the ethanol eluate and concentrate to contain 1.2g of emblica raw material per ml of liquid; the concentrate The aqueous solution is extracted with n-butanol; the n-butanol extraction solution is concentrated to obtain the extract powder of Amla emblica.

[0056] (Ⅱ) Determination of total flavonoids in emblica flavonoids extract

[0057] Adopt the same method of example 1 to measure the flavonoid extract of the emblica flavonoids obtained by the method described in this example, and the...

[0064] (I) Preparation of flavonoids extract from Amla emblica

[0065] Take 1000g of emblica medicinal material, add 7 times the amount of water to extract twice, filter, collect the filtrate, concentrate to 835ml, add hydrochloric acid to adjust the pH to 4-5, and filter; take the acidic filtrate and pass it through a macroporous adsorption resin column. The pore resin model is AB-8, first wash with water until the effluent is neutral; then add 80% ethanol to wash the column; collect the ethanol eluate and concentrate it to contain 0.8g of emblica raw material per milliliter of liquid medicine; concentrate Extract the aqueous solution with n-butanol; concentrate the n-butanol extraction solution to obtain emblica extract powder.

[0066] (Ⅱ) Determination of total flavonoids in emblica flavonoids extract

[0067] Adopt the same method of example 1 to measure the flavonoid extract of the emblica flavonoids obtained by the method described in this example, and the total flavo...