Neutrophil gelatinase associated lipocalin (NGAL) chemiluminescence detection kit

[0042] A chemiluminescence detection kit for neutrophil gelatinase-associated lipocalin, comprising the following components:

[0043] ① Neutrophil gelatinase-associated lipocalin standard product, the neutrophil gelatinase-associated lipocalin referred to as NGAL;

[0044] ② NGAL monoclonal antibody labeled with horseradish peroxidase;

[0045] ③Magnetic particles coated with NGAL monoclonal antibody;

[0046] ④ White opaque microwell plate

[0047] ⑤ washing liquid;

[0048] ⑥ Chemiluminescent substrates A and B;

[0049] Wherein, the magnetic particles coated with NGAL monoclonal antibody are prepared through the following steps:

[0050] ① Take 5.0ml of magnetic particle suspension with a mass content of 2.5% magnetic particles, separate the supernatant from the magnetic plate, and wash the magnetic particles once with deionized water; the liquid in the magnetic particle suspension has a concentration of 0.05mol / L, Phosphate buffer with a pH value of 7.4;

[0051] ②W...

[0073] A chemiluminescence detection kit for neutrophil gelatinase-associated lipocalin, comprising the following components:

[0074] ① Neutrophil gelatinase-associated lipocalin standard product, the neutrophil gelatinase-associated lipocalin referred to as NGAL;

[0075] ② NGAL monoclonal antibody labeled with horseradish peroxidase;

[0076] ③Magnetic particles coated with NGAL monoclonal antibody;

[0077] ④ White opaque microwell plate;

[0078] ⑤ washing liquid;

[0079] ⑥ Chemiluminescent substrates A and B;

[0080] Wherein, the magnetic particles coated with NGAL monoclonal antibody are prepared through the following steps:

[0081] ① Take 5.0ml of magnetic particle suspension with a mass content of 2.5% magnetic particles, separate the supernatant from the magnetic plate, and wash the magnetic particles once with deionized water; the liquid in the magnetic particle suspension has a concentration of 0.05mol / L, Phosphate buffer with a pH value of 7.4;

[0082] ②...

[0103] A chemiluminescence detection kit for neutrophil gelatinase-associated lipocalin, comprising the following components:

[0104] ① Neutrophil gelatinase-associated lipocalin standard product, the neutrophil gelatinase-associated lipocalin referred to as NGAL;

[0105] ② NGAL monoclonal antibody labeled with horseradish peroxidase;

[0106] ③Magnetic particles coated with NGAL monoclonal antibody;

[0107] ④ White opaque microwell plate;

[0108] ⑤ washing liquid;

[0109] ⑥ Chemiluminescent substrates A and B;

[0110] Wherein, the magnetic particles coated with NGAL monoclonal antibody are prepared through the following steps:

[0111] ① Take 5.0ml of magnetic particle suspension with a mass content of 2.5% magnetic particles, separate the supernatant from the magnetic plate, and wash the magnetic particles once with deionized water; the liquid in the magnetic particle suspension has a concentration of 0.05mol / L, Phosphate buffer with a pH value of 7.4;

[0112] ②...