Polymorphic hansenula polymorpha mutant strain and application of polymorphic hansenula polymorpha mutant strain in glutathione biosynthesis
A technology of Hansenula polymorpha and mutant strains, applied in the direction of microorganism-based methods, microorganisms, mutant preparation, etc., can solve the problem of low content, achieve fast division speed, strong viability, and shorten the fermentation cycle.
Inactive Publication Date: 2013-04-24
SHAANXI UNIV OF SCI & TECH
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However, the content of GSH in wild-type Hansenula polymorpha cells is not high
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[0025] The present invention will be further described below in conjunction with the accompanying drawings and embodiments, which are explanations of the present invention rather than limitations.
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Abstract
The invention provides a polymorphic hansenula polymorpha mutant strain and application of the polymorphic hansenula polymorpha mutant strain in glutathione biosynthesis. Firstly, low-energy ions N+ serve as a mutagenesis source, mutagenesis treatment for Hansenula polymorpha DL-1 is achieved, a glutathione (GSH) high-producing strain (DL-18) is obtained, the temperature remains 37 DEG C, the speed remains 180 r / min, shake flask fermentation remains for 42 hours, the dry cell weight (DCW) in end products is 7.04 g / L, the total quantity of the GSH is 420.46 mg / L, and the DCW in the end products and the total quantity of the GSH are respectively improved by 1.73 times and 1.94 times compared with the original mutant strain. Hereditary stability of the DL-18 is detected through subculture, yatsushiro is cultivated, content changes of the GSH are not significant, and therefore, the hereditary stability of the mutant strain is high.
Description
technical field [0001] The invention belongs to the field of industrial microorganisms, and in particular relates to a mutant strain of Hansenula polymorpha induced by low-energy ions and its application in glutathione biosynthesis. Background technique [0002] Glutathione (GSH) is an active tripeptide that widely exists in living organisms. It contains γ-glutamyl and sulfhydryl groups. It can participate in a variety of biological reactions and is important for maintaining a suitable redox environment in living organisms. effect. Because GSH has important physiological functions such as detoxification, anti-oxidation and anti-aging, it has broad application prospects in the fields of clinical medicine, food processing and cosmetics. [0003] Over the years, there have been many studies on the biosynthesis of GSH abroad, and genetically engineered strains have been applied to the production of GSH. At present, the output of GSH basically monopolizes the Chinese market. Co...
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IPC IPC(8): C12N1/16C12N13/00C12N15/01C12P21/02C12R1/645
Inventor 钱卫东付云芳蔡长龙齐香君
Owner SHAANXI UNIV OF SCI & TECH
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