Cell culture plate coated with Matrigel as well as preparation method and application of cell culture plate

[0022] Embodiment 1 is coated with the preparation of the cell culture plate of Matrigel

[0023] (1) Soak polystyrene 6-well cell culture plates in absolute ethanol for 40 min, rinse with deionized water, dry in vacuum, and set aside.

[0024] (2) Measure 50ml of deionized water, add 0.2g of hydroxysuccinimide (NHS) and 0.5g of 2-morpholineethanesulfonic acid hydrate (MES), mix and stir for more than half an hour, filter and sterilize, and store Store at 4°C for later use.

[0025] (3) Weigh 1g of carbodiimide (EDC) and dissolve it in 99ml of deionized water to form an EDC solution with a mass concentration of 1%, filter and sterilize for later use.

[0026] (4) Put the cleaned culture plate into the plasma generator, evacuate to 1Pa, feed oxygen to 50Pa, adjust the discharge power to 60W, and conduct radio frequency discharge for 1min.

[0027] (5) In the culture plate obtained in step (4) under vacuum, add 3ml of the mixed solution prepared in step (2) to each hole, and t...

[0029] Embodiment 2 is coated with the preparation of the cell culture plate of Matrigel

[0030] (1) Soak polystyrene 6-well cell culture plates in absolute ethanol for 40 min, rinse with deionized water, dry in vacuum, and set aside.

[0031] (2) Measure 50ml of deionized water, add 0.1g of hydroxysuccinimide (NHS) and 0.5g of 2-morpholineethanesulfonic acid hydrate (MES), mix and stir for more than half an hour, filter and sterilize, and store Store at 4°C for later use.

[0032] (3) Weigh 5g of carbodiimide (EDC) and dissolve it in 95ml of deionized water to form an EDC solution with a mass concentration of 5%, filter and sterilize it for later use.

[0033] (4) Put the cleaned culture plate into the plasma generator, evacuate to 0.5Pa, feed oxygen to 100Pa, adjust the discharge power to 100W, and conduct radio frequency discharge for 1min.

[0034] (5) In the culture plate obtained in step (4) under vacuum, add 3ml of the mixed solution prepared in step (2) to each hole...

[0036] Embodiment 3 is coated with the preparation of the cell culture plate of Matrigel

[0037] (1) Soak a polystyrene 24-well cell culture plate in absolute ethanol for 40 minutes, rinse with deionized water, dry in vacuum, and set aside.

[0038] (2) Measure 50ml of deionized water, add 0.05g of hydroxysuccinimide (NHS) and 0.5g of 2-morpholineethanesulfonic acid hydrate (MES), mix and stir for more than half an hour, filter and sterilize, and store Store at 4°C for later use.

[0039] (3) Weigh 3g of carbodiimide (EDC) and dissolve it in 97ml of deionized water to form an EDC solution with a mass concentration of 3%, filter and sterilize for later use.

[0040](4) Put the cleaned culture plate into the plasma generator, evacuate to 1Pa, feed oxygen to 200Pa, adjust the discharge power to 10W, and conduct radio frequency discharge for 5min.

[0041] (5) In the culture plate obtained in step (4) under vacuum, add 0.5ml of the mixed solution prepared in step (2) to each ho...