Preparation method of American ginseng extract, and application thereof in preparation of drug for preventing and treating acquired immunodeficiency syndrome

[0018] American ginseng was mixed with acetone 6 times its weight and volume, and extracted 3 times with the tissue crushing extraction method. Each extraction was crushed for 30 seconds, with an interval of 2 seconds, and then crushed for 60 seconds, with an interval of 2 seconds, and then crushed for 30 seconds. Filtrate to obtain the filtrate, combine the filtrates for 3 times, concentrate to dryness under reduced pressure, obtain the residue, dissolve the residue in water with a water weight to volume ratio of 1:1 with Panax ginseng, and through column chromatography equipped with DiaionHP-20 macroporous adsorption resin, First elute with 6 times the column volume of deionized water, then elute with 8 times the column volume of 95% ethanol, collect the ethanol eluate, recover the solvent under reduced pressure to dryness, and obtain the American ginseng extract.

[0020] Add 15 times the weight volume of American ginseng into acetone, and use the tissue crushing extraction method to extract 3 times, each extraction is crushed for 30 seconds, with an interval of 2 seconds, and then crushed for 60 seconds, with an interval of 2 seconds, and then crushed for 30 seconds. Liquid filtration, get filtrate, merge the filtrate of 3 times, concentrate under reduced pressure to dryness, get residue, be 1:1 water dissolving residue with American ginseng water weight volume ratio, through the column chromatography that DiaionHP-20 macroporous adsorption resin is housed , first eluted with deionized water of 6 times the column volume, then eluted with 95% ethanol at a mass concentration of 8 times the column volume, collected the ethanol eluate, recovered the solvent under reduced pressure to dryness, and obtained the American ginseng extract .

[0022] Add 30 times the weight volume of American ginseng into acetone, and use the tissue crushing extraction method to extract 3 times. Each extraction is crushed for 30 seconds, with an interval of 2 seconds, and then crushed for 60 seconds, with an interval of 2 seconds, and then crushed for 30 seconds. Filtrate to obtain the filtrate, combine the filtrates for 3 times, concentrate to dryness under reduced pressure, obtain the residue, dissolve the residue in water with a water weight to volume ratio of 1:1 with Panax ginseng, and through column chromatography equipped with DiaionHP-20 macroporous adsorption resin, First elute with 6 times the column volume of deionized water, then elute with 8 times the column volume of 95% ethanol, collect the ethanol eluate, recover the solvent under reduced pressure to dryness, and obtain the American ginseng extract.