Alchemilla vulgaris extract as well as preparation method and application thereof in resisting wrinkle and whitening

[0027] The preparation of embodiment 1 Kalewort extract

[0028] Take 10 kg of Cottonina basilica, pulverize it, and use 50 L of 90% ethanol aqueous solution to reflux extract 3 times, each time for 3 hours, to obtain 100 L of extract. The extract was concentrated under reduced pressure into an extract (concentration ratio 1:1.2, 60°C) and suspended in water. Then pass through a non-polar macroporous adsorption resin for decolorization, elute with water-ethanol (90:10) to obtain a lighter-colored solution of the extract of Kalejas, concentrate under reduced pressure, and evaporate the solvent to obtain the extract of Kassia.

[0029] The preparation of embodiment 2 Kalewort extract

[0030] Take 10 kg of Cottonina basilica, grind it, and extract it with 50 L of 75% ethanol aqueous solution for 3 times under reflux, each time for 3 hours, to obtain 100 L of extract. The extract was concentrated under reduced pressure into an extract (concentration ratio 1:1.3, 60°C) and suspended in water. Then pass through a non-polar macroporous adsorption resin for decolorization, elute with water-ethanol (90:10) to obtain a lighter-colored solution of the extract of Kalejas, concentrate under reduced pressure, and evaporate the solvent to obtain the extract of Kassia.

[0031] The preparation of embodiment 3 Kalewort extract

[0032] Take 10kg of Cottonina basilica, pulverize it, and use 50L of 95% ethanol aqueous solution to reflux extract 3 times, each time for 3 hours, to obtain 100L of extract. The extract was concentrated under reduced pressure into an extract (concentration ratio 1:1.1, 60°C) and suspended in water. Then decolorize through polar, medium polar or non-polar macroporous adsorption resin, and elute with water-ethanol (90:10) to obtain a lighter-colored kaleba extract solution, which is concentrated under reduced pressure and evaporated to dryness. That is the extract of kale.