Super-resolution fluorescent lifetime imaging method and device based on stimulated emission lost

The invention discloses a super-resolution fluorescent lifetime imaging method based on stimulated emission lost. The super-resolution fluorescent lifetime imaging method based on the stimulated emission lost includes the flowing steps: (1) projecting a first laser beam on a to-be-tested sample and activating grains in the to-be-tested sample to an excited sate; (2) utilizing stimulated emission depletion (STED) light to consume the number of the grains which are in the excited state in step (1); (3) utilizing a second laser beam to stimulate the rest grains to emit fluorescent light and collecting the fluorescent light to obtain corresponding fluorescence intensity images; (4) arranging delay between the second laser beam and the STED light, changing delay time, repeating step (3), and obtaining the fluorescence intensity images under different delay time; (5) conducting treatment on the fluorescence intensity images in different delay time through a computer, and fitting a spot intensity attenuation law, inverting lifetime images, and completing scanning of one point of the to-be-tested sample; (6) and completing two-dimension scanning on the sample to be tested by changing the positions where the beam is projected on the to-be-tested sample. The invention further discloses a device used for implementing the method.