Method for preparing homogeneous and stable solution of tetrahydronaphthalene compounds by using hydroxypropyl-β-cyclodextrin

[0023] 1) Dissolve commercially available hydroxypropyl-β-cyclodextrin in deionized water to prepare hydroxypropyl-β-cyclodextrin with a weight volume (w / v) concentration of 10% (ie 10g / 100mL) solution.

[0024] 2) Weigh 250mg of 8-ring-substituted 2-amino-1,2,3,4-tetralin, and dissolve it in 9ml of the above prepared 10% (w / v) hydroxypropyl-β-cyclodextrin solution In, and dilute to 10ml, the solution is a suspension.

[0025] 3) Dilute the suspension prepared in step 2) to a concentration of 20 mg / ml with 10% (w / v) hydroxypropyl-β-cyclodextrin solution to form a semi-clear solution, which has a small amount of precipitation after standing for 10 minutes .

[0026] 4) Dilute the suspension in step 3) to a concentration of 12.5 mg / ml with 10% (w / v) hydroxypropyl-β-cyclodextrin solution to form a homogeneous and clear solution without precipitation after standing for 10 minutes.

[0027] 5) The prepared 12.5 mg / ml 8-ring substituted 2-amino-1,2,3,4-tetrahydronaphthalene solut...

[0029] 1) Dissolve commercially available hydroxypropyl-β-cyclodextrin in deionized water to prepare hydroxypropyl-β-cyclodextrin with a weight volume (w / v) concentration of 20% (ie 20g / 100mL) solution;

[0030] 2) Weigh 250mg of 8-ring-substituted 2-amino-1,2,3,4-tetralin, and dissolve it in 9ml of the above prepared 20% (w / v) hydroxypropyl-β-cyclodextrin solution In, and dilute to 10ml, the solution is a suspension.

[0031] 3) Dilute the suspension prepared in step 2) to a concentration of 15 mg / ml with 20% (w / v) hydroxypropyl-β-cyclodextrin solution to form a uniform and clear solution, and there is no precipitation after standing for 10 minutes.

[0032] 4) The prepared 15 mg / ml 8-ring substituted 2-amino-1,2,3,4-tetrahydronaphthalene solution can be directly used in related experiments, or diluted to the target concentration for related experiments.

[0034] 1) Dissolve commercially available hydroxypropyl-β-cyclodextrin in deionized water to prepare hydroxypropyl-β-cyclodextrin with a weight volume (w / v) concentration of 30% (ie 30g / 100mL) solution;

[0035] 2) Weigh 250mg of 8-ring substituted 2-amino-1,2,3,4-tetralin and dissolve it in 9ml of the above-prepared 30% (w / v) hydroxypropyl-β-cyclodextrin solution In, and dilute to 10ml, the solution is a suspension.

[0036] 3) Dilute the suspension prepared in step 2) to a concentration of 15 mg / ml with 30% (w / v) hydroxypropyl-β-cyclodextrin solution to form a uniform and clear solution, which has no precipitation after standing for 10 minutes.

[0037] 4) The prepared 15 mg / ml 8-ring substituted 2-amino-1,2,3,4-tetrahydronaphthalene solution can be directly used in related experiments, or diluted to the target concentration for related experiments.