Method of achieving protein expression vector capable of genome site-specific modification

technical field [0001] The invention relates to biological gene cloning and expression technology, in particular to a method for quickly and efficiently constructing a transcription activator-like effector protein cutting enzyme (TALEN) expression vector. It is especially suitable for large-scale construction of expression vectors for genetic modification of higher animal genomes to study the functions of animal genes. Background technique [0002] one. Introduction and application of TALEN technology [0003] With the completion of the whole genome sequence determination of more and more species, the current genomics research hotspot has shifted from structural genomics to functional genomics, so the development of fast, efficient, high-throughput gene function research methods and strategies have a wide range of needs. Gene knockout (Knockout) technology is the most important method in the study of animal gene function. In the past year or so, there have been reports i...