Method for extracting antioxidant ingredient from nigella plant seeds based on cloud point extraction and application of antioxidant ingredient

[0023] In this embodiment, the method for extracting antioxidant components in the seeds of Nigella genus based on the cloud point extraction method includes an extraction step, a cloud point phase-separation extraction step and a water removal step, specifically:

[0024] Step 1: Extraction step

[0025] According to the solid-to-liquid ratio g / mL of 1:20, weigh the defatted Nigella genus seed pulverization, add the surfactant polyoxyethylene glyceryl ether monostearate solution with a concentration of 10g / L, and extract 30 Minutes, filtered to obtain solid filter residue and antioxidant active ingredient extract.

[0026] Step 2: Cloud point phase separation extraction step

[0027] Add sodium chloride to the extract solution of antioxidant active ingredients obtained in the extraction step, adjust the ionic strength to 0.5-1.5mol / L, then place the extract solution in a constant temperature water bath, keep the constant temperature balance for 40 minutes, and let it stand o...

[0033] In this example, the method for extracting the antioxidant components in the seeds of Nigella genus based on the cloud point extraction method includes an extraction step, a cloud point phase-separation extraction step and an elution step, specifically:

[0034] Step 1: Extraction step

[0035] According to the solid-to-liquid ratio g / mL of 1:35, weigh the crushed defatted Nigella genus seeds, add a surfactant Triton X-114 solution with a concentration of 40g / L, ultrasonically assisted extraction for 40 minutes, and filter to obtain a solid Filter residue and antioxidant active ingredient extract.

[0036] Step 2: Cloud point phase separation extraction step

[0037] Add sodium chloride to the extract solution of antioxidant active ingredients obtained in the extraction step, adjust the ionic strength to 0.5-1.5mol / L, then place the extract solution in a constant temperature water bath, keep the constant temperature balance for 60 minutes, and let it stand or centrifug...