Application of ritonavir in preparing medicines for preventing or treating acute lung injury/acute respiratory distress syndrome and pulmonary fibrosis

[0012] Embodiment 2 Ritonavir tablet preparation

[0013] Weigh 100.0g ritonavir and 100.0g carboxymethyl starch sodium, mix well and pass through a 100 mesh sieve, add an appropriate amount of 3% PVP k30 Appropriate amount of water solution to make soft material, granulate with 20-mesh sieve, dry at 60°C for 3 hours, granulate with 18-mesh sieve, add 2.0g of magnesium stearate, mix well and press into tablets, adjust the weight of the tablet to about 200mg.

[0025]Experimental example 2 Effect of ritonavir on acute lung injury / acute respiratory distress syndrome (ALI / ARDS) model rats caused by cecal ligation and perforation

[0026] 2.1 Drugs and reagents

[0027] Ritonavir (purity 99.5%, purchased from Dalian Meilun Biotechnology Co., Ltd.)

[0028] Limulus reagent kit (Fuzhou Xinbei Biochemical Industry Co., Ltd., Fujian Province, batch number: 080430)

[0029] Anti-Aquaporin5 antibody (abcam company, ab104751)

[0030] RAGE primary antibody was purchased from Sigma.

[0031] Experimental animals: SPF grade Sprague Dawley rats, male, weighing 150g-200g, provided by the Experimental Animal Center of Shandong Luye Pharmaceutical Co., Ltd., the animal qualification certificate number is: SYXK (Lu) 20030020.

[0032] 2.2 Experimental methods and results

[0033] 2.2.1 Preparation of rats with cecal ligation and puncture (CLP)

[0034] On the day of the operation, the rats were fasted in the morning, placed in the supine positio...