Method for promoting paeonia suffruticosa seedling growth and application thereof

[0099] Speed ​​up the production of peony seedlings

[0100] 1. Select plump peony seeds, use treatment liquid I [1000mg / L gibberellin (GA 3 )+100ul / L soil temperature (T20) solution] Soak for 24h, discard the liquid, leave the seeds, wash 3 times in warm water, air dry, and place in a refrigerator at 4°C or -20°C for later use.

[0101] 2. Take some clean river sand, sterilize it at 121℃ for 1h, cool it to room temperature, and use treatment liquid II [100mg / L GA 3 +1mg / L6-BA+5mg / L Ca(NO 3 ) 2 +10ul / L T20] Soak in river sand, bury the peony seeds of step (1) in the above wet sand, store them at 4°C in the dark for 2 months, and then root and begin to germinate.

[0102] 3. Sow the rooted peony seeds in step (2) shallowly in a matrix of peat, vegetable garden soil and perlite (3:6:1), and grow them in a greenhouse at 28±2°C and natural light for 1 month , The petiole and leaves are beginning to appear. Bury the peony seeds of step (1) in the above wet sand, store them at 4° C. for 2...

[0107] Speed ​​up the production process of peony tissue culture seedlings

[0108] 1. Select plump peony seeds, use treatment liquid I [1000mg / L gibberellin (GA 3 )+100ul / L soil temperature (T20) solution] Soak for 24h, discard the liquid, leave the seeds, wash 3 times in warm water, air dry, and place in a refrigerator at 4°C or -20°C for later use.

[0109] 2. Peel off the husks of peony seeds obtained in step (1), and use treatment liquid II [100mg / L GA 3 +1mg / L6-BA+5mg / L Ca(NO 3 ) 2 +10ul / L T20] Soak for 24h, discard the liquid, collect seeds with endosperm, filter paper to absorb surface water.

[0110] 3. Wash the endosperm seeds obtained in step (2) with tap water, soak up the surface water droplets, disinfect with 70% ethanol for 30-90 seconds on an ultra-clean workbench, and soak in 0.1% mercury for 5-8 minutes , Rinse with sterile water 5-8 times, absorb water droplets on the sterile material surface with sterile filter paper, peel off the seed embryo, inoculate it in WPM ...