Screening method for glyphosate-resisting medicago sativa plants

[0046] Embodiment 1: a kind of medium screening method of glyphosate-resistant alfalfa plant is characterized in that comprising the steps:

[0047] (1) MS medium to germinate seeds

[0048] Select alfalfa seeds; prepare MS culture plates for later use; carry out seed disinfection under sterile conditions: soak seeds in 100% alcohol for 0.5 min; soak in 2% sodium hypochlorite solution for 15 min; ddH 2 O Wash the seeds 10 times; spread the sterilized seeds evenly on the MS medium with tweezers, seal them with a parafilm and place them in an artificial incubator, 22°C, 18 hours of light, 6 hours of darkness to germinate; the radicle breaks through the testa For germination, the 6th day, the average germination rate reaches more than 50%, stand-by;

[0049] (2) Dose screening

[0050] There are 9 treatments for glyphosate, the concentrations are 0mg / L, 50mg / L, 100mg / L, 150mg / L, 200mg / L, 300mg / L, 500mg / L, 600mg / L, 800mg / L, the configurations are different Concentration of glyp...

[0059] Embodiment 2: a kind of culture medium screening method of glyphosate-resistant alfalfa plant, is characterized in that comprising the steps:

[0060] (1) MS medium to germinate seeds

[0061] Select alfalfa seeds; prepare MS medium culture plates for later use; carry out seed disinfection under sterile conditions: soak seeds in 75% alcohol for 1 min; soak in 2% sodium hypochlorite solution for 10 min; ddH 2 O Wash the seeds 5 times; spread the sterilized seeds evenly on the MS medium with tweezers, seal them with a parafilm and place them in an artificial incubator, 20°C, 16 hours of light, 8 hours of darkness to germinate; the radicle breaks through the seed coat For germination, the 5th day, the average germination rate reaches more than 50%, stand-by;

[0062] (2) Dose screening

[0063] There are 9 treatments for glyphosate, the concentrations are 0mg / L, 50mg / L, 100mg / L, 150mg / L, 200mg / L, 300mg / L, 500mg / L, 600mg / L, 800mg / L, the configurations are different Conce...

[0072] Embodiment 3: a kind of medium screening method of glyphosate-resistant alfalfa plant is characterized in that comprising the steps:

[0073] (1) MS medium to germinate seeds

[0074] Select alfalfa seeds; prepare MS medium culture plates for use; carry out seed disinfection under sterile conditions: soak seeds in 60% alcohol for 2 minutes; soak in 2% sodium hypochlorite solution for 5 minutes; ddH 2 O Wash the seeds 3 times; spread the sterilized seeds evenly on the MS medium with tweezers, seal them with a parafilm and place them in an artificial incubator, 18°C, 0h light, 24h dark to germinate; the radicle breaks through the testa For germination, the 3rd day, the average germination rate reaches more than 50%, stand-by;

[0075] (2) Dose screening

[0076]There are 9 treatments for glyphosate, the concentrations are 0mg / L, 50mg / L, 100mg / L, 150mg / L, 200mg / L, 300mg / L, 500mg / L, 600mg / L, 800mg / L, the configurations are different Concentration of glyphosate medium, ea...