Methods of detecting diseases or conditions

[0405] Representative method for separating phagocytic cells with a DNA content of 2n from non-phagocytic cells and analyzing expression profiles 1

[0406] 1. Separate blood samples into plasma and buffy coat including WBC samples. Plates were coated with avidin to accept WBC samples.

[0407] 2. Add biotinylated antibody to non-phagocytic blood cells (such as T cells) in the wells, incubate at RT for 30 minutes, and wash the wells.

[0408] 3. Add magnetic beads.

[0409] 4. Add WBC blood sample.

[0410] 5. Incubate at 37°C (30 minutes - 1 hour).

[0411] 6. After the beads have been phagocytized by the phagocytes and the avidin-biotin-antibody has bound to the non-phagocytic cells, place the plate on a magnet and wash (phagocytes internalizing the magnetic beads and non-phagocytic cells bound to the antibody will be retained; all other cells will be washed away).

[0412] 7. Remove magnet and collect phagocytic cells and separate into phagocytic cells with DNA equal t...

[0414] Representative method for separating phagocytic cells from non-phagocytic cells II

[0415] 1. Separate blood samples into plasma and buffy coat including WBC samples.

[0416] 2. Cytospin WBC onto glass slides.

[0417] 3. Fix cells in acetone / methanol (-20°C for 5 minutes).

[0418] 4. Stain with hematoxylin and eosin and anti-T cell antibody.

[0419] 5. Isolation of T cells (non-phagocytic) and macrophages (phagocytic) using laser capture microscopy (LCM). Divide into phagocytic cells with DNA equal to 2n and DNA greater than 2n. Non-phagocytic and phagocytic cells with DNA equal to 2n are called cells with DNA equal to 2n.

[0421] Representative method for separating phagocytic cells from non-phagocytic cells III

[0422] 1. Separate plasma from whole blood.

[0423] 2. Isolation of non-phagocytic cells (such as T cells) and phagocytic cells (such as neutrophils and / or macrophages and / or monocytes) from whole blood using magnetic antibody-conjugated beads. Divide into phagocytic cells with DNA equal to 2n and DNA greater than 2n. Non-phagocytic and phagocytic cells with DNA equal to 2n are called cells with DNA equal to 2n.