Sdr5-fc fusion protein and uses thereof

[0049] Acquisition of sDR5 gene:

[0050] The RNA of lung cancer cell lines A549, H1299, SPC-A1 and liver cancer cell line HepG2 were extracted respectively, the sDR5 gene was obtained by RT-PCR technology, and the size of the gene was detected by 2% agarose gel electrophoresis. The results are as follows: figure 1 As shown, the size of the obtained sDR5 gene fragment is in line with expectations, and the sequence of the obtained sDR5 gene is shown in SEQ ID NO:2 after sequencing.

[0052] Sequence overlap of sDR5-Fc DNA fragment and construction of eukaryotic expression vector:

[0053] By overlapping PCR technology, the sDR5 gene sequence (SEQ ID NO: 2) obtained in Example 1 was overlapped with the Fc sequence (SEQ ID NO: 4) to form a sDR5-Fc DNA fragment (SEQ ID NO: 6);

[0054] The sDR5-Fc DNA fragment and the eukaryotic expression vector pcDNA3.1 (+) were digested with Nhe I and Kpn I respectively, and then the sDR5-Fc DNA fragment was inserted into the eukaryotic expression vector pcDNA3.1 (+) with ligase Between the Nhe I / Kpn I, the recombinant vector pcDNA3.1-sDR5-Fc was obtained, and the results were as follows figure 2 As shown, after sequencing, the nucleotide sequence of the recombinant vector is shown in SEQ ID NO:7.

[0055] Recombinant protein expression:

[0056] The recombinant vector pcDNA3.1-sDR5-Fc obtained in Example 2 was transfected into 293T or CHO cells with liposome transfection reagent jetPRIME. After 48 hours, 10 μl of t...