Establishment method for pig unilateral ureter obstruction recanalization model
A technology of ureteral obstruction and establishment method, applied in the field of establishment of animal models, to achieve the effects of easy acquisition, low price, and avoiding repeated stretching
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Embodiment 1
[0028] Include the following steps:
[0029](1) Select experimental animals, the selected experimental pigs are Guangxi Bamaxiang pigs, 3 months old, 8-10 kg in body weight, raised in accordance with standard animal feeding conditions, 18-25 ° C, 12 hours of light / dark cycle, every Pigs are fed pig feed twice a day, 250g each time;
[0030] (2) Modeling operation, the experimental animals were fasted for 12 hours before the operation, and combined anesthesia with diazepam, ketamine and Sumianxin II. The specific operation process of anesthesia: first intramuscularly inject 20 mg / kg of ketamine, and shake the animal after 50 seconds After falling to the ground and muscle relaxation, intramuscular injection of diazepam 1mg / kg and Sumianxin 0.15ml / kg were given intramuscularly, and the anesthesia took effect within 110 seconds. The pig was placed on a constant temperature operating table at 37°C, and all surgical procedures followed aseptic procedures. Routine skin preparation, ...
Embodiment 2
[0034] Include the following steps:
[0035] (1) Select experimental animals, the selected experimental pigs are Guangxi Bamaxiang pigs, 3 months old, 8-10 kg in body weight, raised in accordance with standard animal feeding conditions, 18-25 ° C, 12 hours of light / dark cycle, every Pigs are fed pig feed twice a day, 250g each time;
[0036] (2) Modeling operation, the experimental animals were fasted for 12 hours before the operation, and combined anesthesia with diazepam, ketamine and Sumianxin II. The specific operation process of anesthesia: first give ketamine 20mg / kg intramuscular injection, and shake the animal after 60 seconds After falling to the ground and muscle relaxation, intramuscular injection of diazepam 1mg / kg and Sumianxin 0.15ml / kg were given intramuscularly, and the anesthesia took effect within 120 seconds. The pig was placed on a constant temperature operating table at 37°C, and all surgical procedures followed aseptic procedures. Routine skin preparatio...
Embodiment 3
[0040] Include the following steps:
[0041] (1) Select experimental animals, the selected experimental pigs are Guangxi Bamaxiang pigs, 3 months old, 8-10 kg in body weight, raised in accordance with standard animal feeding conditions, 18-25 ° C, 12 hours of light / dark cycle, every Pigs are fed pig feed twice a day, 250g each time;
[0042] (2) Modeling operation, the experimental animals were fasted for 12 hours before the operation, and combined anesthesia with diazepam, ketamine and Sumianxin II. The specific operation process of anesthesia: first intramuscularly inject 20 mg / kg of ketamine, and shake the animal after 70 seconds After falling to the ground and muscle relaxation, intramuscular injection of diazepam 1mg / kg and Sumianxin 0.15ml / kg were given intramuscularly, and the anesthesia took effect within 130 seconds. The pig was placed on a constant temperature operating table at 37°C, and all surgical procedures followed aseptic procedures. Routine skin preparation,...
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