A kind of preparation method of cross-linked sodium hyaluronate gel with asymmetric structure

[0020] Prepare 20ml of 1% sodium hydroxide aqueous solution, add 0.02g of cross-linking agent BDDE, mix well, add 2.0g sodium hyaluronate, stir well, put it in a 10°C reaction box, set the dehydration rate to 3% / day , after reacting for 10 days, put it into a 40°C water bath, heat for 2 hours, take out the gel cut into about 0.5g in mass, put it in PBS buffer solution, and perform swelling dialysis to obtain cross-linked sodium hyaluronate gel. 20mg / ml.

[0022] Prepare 20ml of 1% sodium hydroxide aqueous solution, add 0.01g of cross-linking agent BDDE, mix well, add 2.0g of sodium hyaluronate, stir well, put it in a 10°C reaction box, set the dehydration rate to 4% / day , after reacting for 10 days, put it into a 40°C water bath, heat for 2 hours, take out the gel cut into about 0.5g in mass, put it in PBS buffer solution, and perform swelling dialysis to obtain cross-linked sodium hyaluronate gel. 20mg / ml.

[0024] Prepare 20ml of 1.5% sodium hydroxide aqueous solution, add 0.007g of cross-linking agent BDDE, mix well, add 2.0g of sodium hyaluronate, stir well, put it in a 10°C reaction box, set the dehydration rate to 5% / day , after reacting for 10 days, put it into a 40°C water bath, heat for 2 hours, take out the gel cut into about 0.5g in mass, put it in PBS buffer solution, and perform swelling dialysis to obtain cross-linked sodium hyaluronate gel. 20mg / ml.