Application of Teprenone in preparing drug for preventing and/or treating readdiction in opiates narcotics

[0015] Example 1: Teprenone antagonizes morphine-induced conditioned place preference experiment

[0016] The small animal autonomous activity detection device was used to measure the mice, and the mice were divided into 4 groups, with 10 mice in each group. The control group (C) was intragastrically administered normal saline for 7 consecutive days; the morphine group (Mor) was intragastrically administered normal saline for 7 consecutive days; the GGA and morphine-GGA group (GGA+Mor) was intragastrically administered GGA (800 mg / kg) for 7 consecutive days . On the eighth day, the initial time of the conditioned place preference experiment was measured for 15 minutes. Then on the 9th, 11th, 13th, and 15th day respectively, group C received intraperitoneal injection of normal saline, GGA and GGA+Mor group received intragastric administration of GGA (800 mg / kg), Mor group and GGA+Mor group received intraperitoneal injection of morphine 20 mg / kg, on the 10th, 12th, 14th, an...

[0017] Example 2: Teprenone inhibits the increased expression of NR2B in the NAc region induced by morphine relapse

[0018] Tissue collection and storage: After the mice were sacrificed by cervical dislocation, the chest was opened, the venous vessel end of the right atrium was cut open, and the left ventricle was perfused with 1×PBS buffer solution at a flow rate of 20 rpm. Bai Hou stopped. Then the tissues in the NAc area were taken, divided into liquid nitrogen, quick-frozen, marked, and stored in a -80°C refrigerator.

[0019] Extraction of protein in brain tissue: Take out the tissue in the NAc area frozen in the -80 ℃ refrigerator, use tweezers to take out the tissue of the same part and the same size, put it into a glass grinder and grind it into dispersed cells, add an appropriate amount of cell lysis Then suck out the mixed solution and put it into a 1.5 ml eppendorf centrifuge tube, and then crack it in ice bath every 10 min for 50 min, centrifuge at 15000 rpm an...