Method and related biological material for cultivating transgenic plant of fluorescent protein mCherry labeled microtubulin

[0053] Embodiment 1, the expression vector pEarleyGate302-P used in the method for cultivating the transgenic plant of fluorescent protein mCherry labeling tubulin TUB6 :: Preparation of mCherry-TUB6 and its related biomaterials

[0054] The name shown in the artificially synthesized SEQ ID No.1 is P TUB6 :: DNA molecule of mCherry-TUB6. In SEQ ID No.1, positions 1-3004 are promoter P TUB6 ; The 3005-5074th position of SEQ ID No.1 is the mCherry-TUB6 fusion gene, the coding sequence of the mCherry-TUB6 fusion gene is the 3005-5074th position of SEQ ID No.1, encoding the fusion shown in SEQ ID No.2 Protein mCherry-TUB6; No. 3005-3712 of SEQ ID No.1 is the coding gene of mCherry, which encodes mCherry shown in No. 1 to No. 236 of SEQ ID No.2; No. 3713-3713 of SEQ ID No.2 Position 3724 is the coding gene of GA-Linker, which encodes the GA-Linker shown in the 237-240th position of SEQ ID No.2; the 3725-5074th position of SEQ ID No.2 is the coding gene of plant tubulin TUB6 , w...

[0060] Embodiment 2, the method for cultivating the transgenic plant of fluorescent protein mCherry labeling tubulin

[0061] 1.P TUB6Transfected pEarleyGate302-P that initiates the expression of mCherry-TUB6 fusion protein TUB6 :: Preparation of mCherry-TUB6 Arabidopsis (using pEarleyGate302-P TUB6 :: mCherry-TUB6 to cultivate transgenic plants with fluorescent protein mCherry-labeled tubulin)

[0062] Arabidopsis transformation was done by flower dipping method. Activated GV3101 / pEarleyGate302-P TUB6 :: mCherry-TUB6 was cultured in shake flask at 28°C for 24 hours in a final concentration of 50mg / mL kanamycin and 14mg / mL gentamicin in 5mL YEP liquid medium; after 200mL expansion culture, centrifuge at 4°C at 3600rpm for 15min to collect Bacterial cells were resuspended with 5% sucrose solution, and silwet L-77 with a volume ratio of 0.05% was added to obtain a bacterial suspension; the Colombian ecotype Arabidopsis thaliana inflorescences that had grown to the full flowe...