Use of Daphnilongeranin A in preparation of monoamine oxidase (MAO) inhibitor

[0011] Embodiment 1: the preparation of the compound DaphnilongeraninA tablet involved in the present invention:

[0012] Get 20 grams of compound Daphnilongeranin A and add 180 grams of conventional excipients for preparing tablets, mix well, and make 1000 tablets with a conventional tabletting machine.

[0013] Embodiment 2: the preparation of compound DaphnilongeraninA capsule involved in the present invention:

[0014] Get 20 grams of compound Daphnilongeranin A, add conventional auxiliary materials for preparing capsules, such as 180 grams of starch, mix well, and make 1000 capsules.

[0015] The following pharmacodynamic experiments will further illustrate its drug activity.

[0016] Compound DaphnilongeraninA was tested for its inhibitory activity on MAO-A and MAO-B by fluorescent probe method,

[0017] The described method proceeds according to the following steps:

[0018] Dissolve the sample to be tested in DMSO to prepare a series of samples with concentration gradient. Take 4ul of MAO (10mg / ml), boric acid buffer (PH=8.4), 50ul of BSA (50mg / ml), add the sample solution to be tested, mix well, react in a water bath at 30-38°C for 3h, then add probe 7- (3-Aminopropoxy)coumarin 2ul (50mmol / ml), the final concentration of the inhibitor is 0~10-2mmol / l, the mixture is ...