Application of polypeptide hydrogel serving as protein vaccine adjuvant and protein vaccine

[0020] (1) According to Document 1, the L-configuration phosphorylated polypeptide Nap-GFFpY-OMe was synthesized by FMOC-solid-phase synthesis method, and its nuclear magnetic spectrum and mass spectrometry results are as follows:

[0021] 1 HNMR (400MHz, DMSO-d 6 )δ8.48(d,1H,NH),8.19-8.28(m,2H,NH),8.06(d,1H,NH),7.75-7.88(m,3H),7.75(s,1H),7.41- 7.48(m,3H),7.07-7.25(m,14H),4.46-4.58(m,3H),3.70-3.76(m,1H),3.6-3.68(s,4H),3.58(s,3H), 2.92-3.03(m,4H),2.76-2.81(m,1H),2.63-2.69(m,1H).MS:calc.M + =794.3, obsvd.(M+Na) + = 817.3.

[0022] (2) Take 1mgL configuration phosphorylated polypeptide Nap-GFFpY-OMe and place it in a 1.5ml glass bottle, add 150 microliters of PBS (pH=7.0) solution, adjust its pH value to 7.0 with sodium carbonate solution, at 20 Add 2 microliters of 2U / microliter alkaline phosphatase at ~25°C, set the volume to 250 microliters with PBS solution, let it stand for about 10 minutes to form a gel, and wait until the reaction is basically complete (about 2 hours)...

[0025] (1) Use the amino acid raw material of D configuration instead, according to literature 1, synthesize D configuration phosphorylated polypeptide Nap-GFFpY-OMe with FMOC-solid-phase synthesis method, its nuclear magnetic spectrum and mass spectrometry results are as follows:

[0026] 1 HNMR (300MHz, DMSO-d 6 )δ8.50(d,1H,NH),8.16-8.24(m,2H,NH),8.10(d,1H,NH),7.72-7.85(m,3H),7.74(s,1H),7.40- 7.46(m,3H),7.08-7.24(m,14H),4.47-4.59(m,3H),3.72-3.75(m,1H),3.58-3.68(s,4H),3.60(s,3H), 2.90-3.04(m,4H),2.73-2.85(m,1H),2.62-2.68(m,1H).MS:calc.M+=794.3,obsvd.(M+H) + =795.3.

[0027] (2) Take 1 mg D-configuration phosphorylated polypeptide Nap-GFF p Put Y-OMe in a 1.5 ml glass bottle, add 150 microliters of PBS (pH=7.0) solution, adjust the pH value to 7.0 with sodium carbonate solution, add 2 microliters of 2U / microliter at 20-25°C Alkaline phosphatase was fixed to 250 microliters with PBS solution, gelled after standing for about 10 minutes, and the polypeptide hydrogel was obta...