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Bactericidal compound and preparation method thereof

A compound and bactericide technology, applied in the field of bactericidal compounds and their preparation

Active Publication Date: 2016-05-04
HAINAN UNIVERSITY
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Problems solved by technology

[0004] The target strain of this study is Bacillus amyloliquefaciens HAB-2 (the strain was preserved in the China Type Culture Collection Center on January 27, 2015, and proved to be alive. Its preservation registration number is: CCTCCM2015070, and the address is in Wuhan University) A series of preliminary experiments showed that the biocontrol bacterium HAB-2 has a broad-spectrum activity of inhibiting tropical pathogenic fungi, and can promote the growth of some plants and keep fresh fruits. However, this strain does not have the sfp gene, but through modern With the instrumental analysis method, we have detected the lipopeptide substances—surfactin substances that cannot be obtained due to the lack of sfp in the HAB-2 fermentation broth. Therefore, it is necessary to study the specific active ingredients of this strain, and it is worth further research. Research

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  • Bactericidal compound and preparation method thereof
  • Bactericidal compound and preparation method thereof
  • Bactericidal compound and preparation method thereof

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Embodiment Construction

[0033] Referring to the accompanying drawings, the present invention will be further described in conjunction with specific embodiments, so as to better understand the present invention.

[0034] 1. Compound separation

[0035] 1.1 Fermentation broth of Bacillus amyloliquefaciens HAB-2

[0036] Inoculate the preserved biocontrol bacteria strain HAB-2 on the lysing broth medium for activation at 28°C, cultivate for 3 days, pick out the soybean grain-sized biocontrol bacteria HAB-2 and add it to the liquid lysing broth medium, Shake the bacteria at 28°C and 200 rpm for 2 days to obtain the fermentation broth. Use a high-speed centrifuge and centrifuge at 10,000 rpm for 10 minutes to remove the bacteria and obtain a sterile fermentation broth.

[0037] 1.2 Butanol extraction method

[0038] Fully mix the fermented liquid and n-butanol at a ratio of 1:1 by volume, stand still for 12 hours, extract with a separatory funnel to obtain n-butanol in the upper layer, and use a rotary ...

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Abstract

The invention provides a bactericidal compound. The structural formula of the bactericidal compound is shown in the specification. According to the invention, specific active ingredients of a fermentation broth of bacillus amyloliquefaciens HAB-2 are studied, and separated to obtain a modified bactericidal compound, and the modified bactericidal compound has broad-spectrum activity of inhibiting tropical pathogenic fungi, and the effects of promoting growth of part of plants and preserving fresh fruits, thereby being worthy of further research.

Description

technical field [0001] The invention belongs to the field of compounds, and in particular relates to a bactericidal compound and a preparation method thereof. Background technique [0002] Bacillus is a kind of Gram-positive strain that produces spores. It is widely distributed, rich in physiological characteristics, easy to isolate and cultivate, and is an important microbial flora in nature. Bacillus can produce a variety of antibacterial active substances, including Non-ribosomal peptide antibiotics synthesized by ribosomal pathway and bacteriocins, enzymes and other active protein antibacterial substances synthesized by ribosomal pathway. [0003] Strain 168 is the model strain of Bacillus and the most thoroughly studied strain at present. Its whole genome was sequenced in 1997. Different from the wild Bacillus, the model strain 168 lost many wild-type characteristics due to the long-term subculture in the laboratory, such as the spontaneous mutation of the sfp gene, wh...

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Application Information

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IPC IPC(8): C07D487/04A01N43/90C12P17/18A01P3/00C12R1/07
CPCA01N43/90C07D487/04C12P17/182
Inventor 缪卫国靳鹏飞王皓楠刘文波郑服丛谢清标
Owner HAINAN UNIVERSITY
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