Application of Shixiantao polysaccharide in the preparation of liver-protecting medicine

The invention discloses application of pholidota chinensis lindl. polysaccharides to preparation of hepatoprotective. Pseudobulbs or whole herb of collected fresh pholidota chinensis lindl. is dried in shade and crushed to obtain coarse pholidota chinensis lindl. powder, water is added according to a material-liquid ratio of 1:10, extraction is performed twice with 1.5h each time by virtue of boiling water, extracting solutions are mixed for concentration, extraction is performed many times with chloroform and n-butanol in a proportion of 4:1 by adopting a Sevag method to obtain supernatant in which proteins are removed, 95 percent ethanol is added to make the ethanol content reach 80 percent, ethanol precipitation is performed on the mixed solution in a 4 DEG C refrigerator for 12h, suction filtration is performed, and filter residues are washed sequentially with ethyl ether, acetone and anhydrous ethanol to obtain crude pholidota chinensis lindl. polysaccharides; the crude pholidota chinensis lindl. polysaccharides are dissolved in water, the obtained solution is loaded into a DE52 type resin chromatographic column subjected to pretreatment with a phosphate buffer solution with pH of 7.5, stands overnight, and is eluted with distilled water at the flow velocity of 1.2ml / min, and eluent is combined and freeze-dried to obtain the pholidota chinensis lindl. polysaccharides. The liver protecting effects of the pholidota chinensis lindl. polysaccharides are developed, and a research foundation is laid for further deep development of the pholidota chinensis lindl. polysaccharides.