TK, ge and gT gene deletion strain of porcine pseudorabies virus variant and application of TK, ge and gT gene deletion strain

[0044] The present invention is further illustrated by the following examples.

[0045] 1 Materials and methods

[0046] 1.1 Main materials

[0047] 1.1.1 Viruses and Cells

[0048] PRV ZJ01 strain virus, which was isolated by our laboratory in February 2012 from sick piglets in a large-scale pig farm in Zhejiang, is a highly pathogenic strain and is adapted to the F8 generation of BHK-21 cells; PRV ZJ01ΔgE / gI strain was used in this experiment The gI and gE double-gene deletion virus obtained by using the PRV ZJ01 strain as a parent was adapted to the F10 generation of BHK-21 cells. BHK-21 cells were preserved by our laboratory, the cell growth medium was DMEM (Gibco) containing 10% fetal bovine serum (Gibco), and the maintenance medium was DMEM containing 2% fetal bovine serum.

[0049] 1.1.2 Plasmids and strains

[0050] pHA2 plasmid (including BAC vector sequence, gfp expression cassette expressed in eukaryotic cells, chloramphenicol resistance gene expressed in Escher...