A kit for screening cancer brain metastases

[0025] Example 1 The relationship between the expression level of long non-coding RNA lnc-MMP2-2 and patients with brain metastases from lung cancer

[0026] 1. Experimental materials

[0027] Lung adenocarcinoma tissue chip microarray (XT14-051): purchased from Shanghai Xinchao Biotechnology Co., Ltd., including 19 cases of normal lung tissue, 14 cases of in situ lung adenocarcinoma tissue (non-metastasis) and brain metastasis tissue of lung adenocarcinoma 11 cases;

[0028] FAM-lncRNA-MMP2-2 nucleic acid probe was designed and synthesized by Shanghai Gefan Biotechnology Co., Ltd., the sequence is: 5'-FAM-ACCCTAGGCTGCAGGCTCCTGCTTTGGGCT-3';

[0029] In situ hybridization kit (C004) was purchased from Shanghai Gefan Biotechnology Co., Ltd., the kit contains: 10X proteinase K, prehybridization solution, acetic anhydride, triethanolamine aqueous solution pH8.0, 20XSSC pH4.5, 20X SSC pH7.5 ;

[0030] Other self-prepared reagents (glycine, formamide, hydrochloric acid, xylene, 3...

[0060] Example 2 The composition and method of use of the kit for detecting the expression level of long-chain non-coding RNA lnc-MMP2-2 according to the present invention

[0061] 1. The composition of the kit

[0062] FAM-lncRNA-MMP2-2 nucleic acid probe, the sequence is:

[0063] 5'-FAM-ACCCTAGGCTGCAGGCTCCTGCTTTGGGCT-3';

[0064] For in situ hybridization: 10X proteinase K, prehybridization solution, acetic anhydride, triethanolamine aqueous solution pH8.0, 20XSSC pH4.5, 20X SSC pH7.5.

[0065] DEPC water and DAPI dye, antifade mounting medium.

[0066] 2. How to use the kit

[0067] The brain tissue of the sample to be tested was used to detect the expression level of lncRNA-MMP2-2 according to the in situ hybridization method.

[0068] For lung cancer patients with suspected metastases, tissue from abnormal parts of the brain and lung tissue can be collected to compare the expression level of lncRNA-MMP2-2, and then evaluate the possibility of lung cancer brain metast...