Supercharge Your Innovation With Domain-Expert AI Agents!

A kind of establishment method of type 2 diabetes animal model

A type 2 diabetes, animal model technology, applied in pharmaceutical formulations, resistance to vector-borne diseases, medical preparations containing active ingredients, etc. The effect of short molding cycle, easy operation and low cost

Inactive Publication Date: 2020-01-31
UNIV OF ELECTRONICS SCI & TECH OF CHINA
View PDF1 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method takes a long time to induce. Taking male C57BL / 6J mice as an example, after feeding high-fat diet for 4 to 12 months, they showed abnormal glucose tolerance and compensatory increase in blood insulin level; The influence of the dose of STZ single injection, the palatability of high-fat feed is worse than that of ordinary feed, animals often fail to make models due to reduced food intake, and inappropriate STZ single injection dose will prolong the modeling cycle and increase animal mortality

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of establishment method of type 2 diabetes animal model
  • A kind of establishment method of type 2 diabetes animal model
  • A kind of establishment method of type 2 diabetes animal model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0035] The present invention is mainly used for establishing type 2 diabetes animal model, and it is based on common feed, lipopolysaccharide (derived from Escherichia coli 0111: B4 subspecies), normal saline, streptozotocin (STZ), and Rats were injected to realize the establishment of type 2 diabetes animal model. Operation process of the present invention is as follows:

[0036] (1) Dissolving lipopolysaccharide with physiological saline to prepare a lipopolysaccharide solution with a concentration of 100 μg / ml; the prepared lipopolysaccharide solution is subpackaged and stored at -20°C, and taken out when used to avoid repeated freezing and thawing;

[0037] (2) Select 4-6 week-old male mice and feed them with common feed. At the same time, inject lipopolysaccharide solution once a day for a total of 4 weeks. The dosage is 500 μg / kg / d. Injection is given by subcutaneous continuous injection using a pump;

[0038] (3) Observe the feeding and activity situation of the male ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for establishing an animal model of type 2 diabetes. The steps are as follows: (1) prepare lipopolysaccharide solution with physiological saline, and the concentration is 100 μg / ml; Feed and inject lipopolysaccharide solution at the same time; (3) measure the body weight of the male mice after the 28th day; (4) fast the male mice for 8 hours after measuring the body weight, and separate serum to measure blood sugar and insulin; (5) evaluate insulin Resistance ability and evaluation of glucose-stimulated insulin secretion ability; (6) On the 30th day and the 32nd day, the male mice were given intraperitoneal injection of STZ solution once; (7) When the random blood sugar> 16.8mmol / L, the type 2 diabetic animal was determined The model is built. The present invention skillfully selects the lipopolysaccharide in Escherichia coli 0111:B4 subspecies, cooperates with common feed feeding and STZ injection, and successfully establishes the type 2 diabetes animal model. The invention is easy to operate, and the cost of building a model is low, and the molding rate is high and the molding cycle is short.

Description

technical field [0001] The invention relates to the field of animal models of diabetes, in particular to a method for establishing an animal model of type 2 diabetes. Background technique [0002] Diabetes mellitus (DM) is one of the most challenging public health problems in the world. According to the report of the International Diabetes Federation (IDF), there are about 382 million diabetic patients in the world, and it is expected to grow to 592 million by 2035, of which 80% to 85% are type 2 diabetes (type 2diabetes, T2DM). The prevalence of diabetes in China has already ranked first in the world and continues to rise, from 0.67% (1980), 9.7% (2007), to 11.6% (2013). At present, there are 113.9 million adult patients with diabetes in my country, of which type 2 diabetes (T2DM) Accounted for the vast majority. T2DM has a variety of complications (obesity, hypertension, hyperlipidemia), which can easily lead to chronic complications such as coronary heart disease, stroke...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/739A01K67/02
CPCA01K67/02A61K31/739Y02A50/30
Inventor 孙勤
Owner UNIV OF ELECTRONICS SCI & TECH OF CHINA
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com