Method for detecting flumazenil in Chinese patent medicines and health food by liquid chromatography-mass spectrometry

[0020] (1) Preparation of reference substance solution: take the flumazenil reference substance and dissolve it with methanol to prepare a flumazenil solution with a concentration of 1mg / ml, then take 1ml of the flumazenil solution in a measuring bottle, and dilute to 1 mg / ml with methanol 100ml to obtain the flumazenil reference substance solution.

[0021] (2) Prepare the sample solution to be tested: the configuration method of the liquid preparation: accurately draw a considerable oral dose, put it in a 50ml measuring bottle, add 20ml of 50% aqueous methanol solution, and ultrasonically process it for 15min at 1130W under the condition of 37kHz, let it cool, and use Dilute 50% aqueous methanol to the mark, shake well, take 1ml of the solution and dilute it 10 times with water, and use it as the test solution for later use.

[0022] The preparation method of the solid preparation: take the test product of the solid preparation and grind it finely, accurately weigh the dose ...