Purpose of coumarin compound in preparation of monoamine oxidase inhibitor

[0022] Embodiment 1: the test of compound of the present invention to monoamine oxidase inhibitory activity

[0023] 1. Experimental reagents

[0024] Compounds of the present invention, MAO-A (Active Motif, Cat.No.31502), MAO-B (Active Motif, Cat.No. 31503), Clorgyline (Sigma, Cat.No.M3778), Sylvia Gillan (R(-)-deprenyl) (Abcam, Cat. No. ab120604), 384-well plate (Perkin Elmer, Cat. No. 6007299).

[0025] 2. Experimental method

[0026] 1) Dissolve the compound of the present invention to 10 mM with 100% DMSO, add HEPES as a buffer in the multi-well plate, transfer the compound to the multi-well plate, and reduce the concentration of DMSO to 1%.

[0027] 2) Dissolve the enzyme and the substrate in the buffer solution respectively, transfer 10 μL of the enzyme solution into the multi-well plate, and let it stand for 15 minutes to room temperature.

[0028] 3) Add 10 μL of substrate solution, start the reaction, let it stand for 60 minutes, add 20 μL of fluorescein, mix well...