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H-E dyeing liquid for pathological tissue dyeing machine

A technology of staining solution and dyeing machine, applied in the field of H-E staining solution, which can solve the problems of complex preparation of H-E staining solution, affecting the accuracy of pathological reading, and unstable staining effect, etc., to achieve clear cell structure, bright cell coloring, and easy promotion Effect

Inactive Publication Date: 2019-02-26
武汉宏强医疗器械有限公司
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AI Technical Summary

Problems solved by technology

[0008] The problem to be solved by the present invention is that the preparation of the traditional H-E staining solution is relatively complicated, the staining solution is easy to be old, and the dyeing effect is unstable, and the effect is not good when used with a highly automated pathological tissue staining machine, which affects the accuracy of the doctor's pathological reading. , affecting the diagnosis of the disease

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1, the preparation method of H-E staining solution of the present invention is:

[0021] For every 1000ml of improved hematoxylin staining solution, sodium iodate 0.04g; hematoxylin color essence 3g; aluminum potassium sulfate 28g; absolute ethanol 260ml; formaldehyde 0.1g; Stir to dissolve.

[0022] Further, every 1000ml of improved eosin staining solution includes the following components: 1.2g of alcohol-soluble eosin, 200ml of absolute ethanol, 45g of potassium aluminum sulfate, 0.5g of sodium iodate, 6g of water-soluble eosin, and 0.5g of flamingo , 10ml of glacial acetic acid, 0.2g of formaldehyde, and deionized water as the balance, let the above components be evenly stirred and dissolved.

[0023] The H-E staining solution prepared by the above examples reduces the cost of use and saves the time for preparing samples during use. The H-E staining solution provided is matched with the pathological tissue staining machine for uniform staining, and the p...

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Abstract

The invention provides an H-E dyeing liquid for a pathological tissue dyeing machine, and relates to the technical field of biological preparations. The H-E dyeing liquid comprises modified hematoxylin dyeing liquid and modified eosin dyeing liquid, wherein the modified hematoxylin dyeing liquid comprises following components: sodium iodate, hematoxylin, aluminum potassium sulfate, anhydrous ethanol, formaldehyde, glacial acetic acid, and water; and the modified eosin dyeing liquid comprises following components: alcohol-soluble eosin, anhydrous ethanol, aluminum potassium sulfate, sodium iodate, water-soluble eosin, erythrosine, glacial acetic acid, formaldehyde, and deionized water. The using cost is reduced, the sample preparation time is shortened, the provided H-E dyeing liquid can evenly dye pathological tissues under the assistance of a pathological tissue dyeing machine, the structure of dyed pathological tissues is clear, the color of cells is bright, and the observation on slides of pathological tissues becomes easy in clinic.

Description

technical field [0001] The invention relates to the technical field of biological preparations; in particular, it relates to an H-E staining solution used in a pathological tissue staining machine. Background technique [0002] At present, the incidence of serious diseases such as various cancers, tumors, and tissue lesions is on the rise. How to effectively diagnose these diseases has gradually become a hot spot in pathological research. [0003] Pathological tissue preparation technology can assist clinicians to detect the above-mentioned diseases early and formulate correct treatment plans, so that patients can recover as soon as possible and reduce pain, so it has attracted the attention of researchers and clinicians. [0004] One of the important steps in pathological tissue preparation technology is to stain the pathological tissue, and H-E staining solution is one of the most commonly used staining methods. The sample cells stained with H-E staining solution have goo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/30
CPCG01N1/30G01N2001/302
Inventor 不公告发明人
Owner 武汉宏强医疗器械有限公司
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