Multiplex PCR method capable of simultaneously detecting 7 virulence genes of Aeromonas

The invention provides a multiplex PCR method capable of simultaneously detecting 7 virulence genes of Aeromonas. The invention comprises the following steps: screening of primer combination and primer sequences; a preparation method of pathogenic bacteria DNA template; multiplex PCR reaction system and PCR amplification; and multiplex PCR product detection and result interpretation. The multiplexPCR detection method can efficiently, specifically and sensitively detect and identify the presence of Aeromonas in a sample and 1-7 virulence genes through two PCR reactions. The method is applicable to the rapid identifying of pathogenic strains and common strains of Aeromonas fish, and is applicable to molecular-assisted diagnosis of fish Aemmonasis and confirmed diagnosis of such disease. Themethod is applicable to the whole process monitoring of aquaculture animal breeding, is applicable to different sample types including water bodies, sediments, farm animals and aquatic products, monitors whether the sample contains Aeromonas or not and that the strain is common or pathogenic Aeromonas, and can identify the virulence gene types and relative expression levels of pathogenic Aeromonas.