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Respiratory syncytial virus F expressing protein and preparation method thereof

A technology of syncytial virus and respiratory tract, which is applied in the field of expression of respiratory syncytial virus F protein and its preparation, can solve problems such as difficulty in development, achieve structural instability, maintain integrity, and increase the probability of positive antibodies

Inactive Publication Date: 2019-04-30
苏州高泓利康生物科技有限公司
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Problems solved by technology

At present, there is no applicable RSV vaccine in the world, and its development is a worldwide problem

Method used

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  • Respiratory syncytial virus F expressing protein and preparation method thereof
  • Respiratory syncytial virus F expressing protein and preparation method thereof
  • Respiratory syncytial virus F expressing protein and preparation method thereof

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Embodiment Construction

[0024] The present invention discloses a method for expressing respiratory syncytial virus F protein and its preparation method, wherein the amino acid sequence of the F protein is SEQ:ID:NO:1, and its sequence is:

[0025] MELPILNTNAITTILAAVTLCFASSQNITEEFYQSTCSAVSKGYLSALRTGWYTSVITIELSNIKENKCNGTDAKVKLIKQELDKYKNAVTELQLLMQSTPAANSRARREAPRGMRYTMNLQRNVNVTDSLKRKRRFLGFLLGVGSAIASGIAVSKVLHLEGEVNKIKSALLSTNKAVVSLSNGVSVLTSKVLDLKNYIDKQLLPIVNKQSCSISNIETVIEFQQKNNRLLEITREFSVNAGVTTPVSTYMLTNSELLSLINDMPITNDQKKLMSNNVQIVRQQSYSIMSIIKEEVLAYVVQLPLYGVIDTPCWKLHTSPLCTTNTKEGSNICLTRTDRGWYCDNAGSVSFFPQAETCKVQSNRVFCDTMNSLTLPSEVNLCNIDIFNPKYDCKIMTSKTDVSSSVITSLGAIVSCYGKTKCTASNKNRGIIKTFSNGCDYVSNKGVDTVSVGNTLYYVNKQEGKSLYVKGEPIINFYDPLVFPSDEFDASISQVNEKINQSLAFIRKSDELLHNVNAGKSTTNIMITTIIIVIIVILLSLIAVGLLLYCKARSTPVTLSKDQLSGINNIAFSN

[0026] The preparation method comprises the following steps: selecting the extracellular segment (26AA-513AA) of the RSV viral F protein from the full-length sequence of the RSV viral F protei...

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Abstract

The invention provides a respiratory syncytial virus F expressing protein and a preparation method thereof, wherein the F protein has the amino acid sequence of SEQ ID NO:1. Amino acid point mutationis carried out at a Furin recognition site of the soluble F protein; at the same time, a GCN4 fragment is added at a C end to prepare the F protein; through the sequence design, the F protein expressed in an eukaryotic system is guaranteed to avoid being enzymatically hydrolyzed by Furin so as to maintain the integrity of an extracellular fragment and the Pre-fusion state and complete immunogenicity of the F protein are maintained. The F protein is necessary to induce a body to produce antibodies with immunoprotective effect against RSV, and effectively improves the probability to screen positive antibodies in the later stage.

Description

technical field [0001] The invention relates to an expression of F protein of respiratory syncytial virus and a preparation method thereof, belonging to the technical field of biomedicine. Background technique [0002] Respiratory syncytial virus (RSV) belongs to the Pneumovirus genus of the Paramyxoviridae family, and is an enveloped single-stranded negative-sense RNA virus. RSV virus causes about 64 million global infections every year, more than 3 million infants are hospitalized, and 160,000 infants die. It is the most important pathogen that causes viral bronchitis and pneumonia in infants and young children. Therefore, prevention of RSV infection Immunization and clinical treatment are extremely important. Currently, there is no applicable RSV vaccine in the world, and its development is a worldwide problem. Therefore, the lower respiratory tract disease caused by RSV virus has become one of the most seriously missing diseases in the planned immunization of infants a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/135C12N15/45C12N15/85
CPCC07K14/005C12N15/85C12N2760/18522C12N2800/22
Inventor 高光崔院平程远政
Owner 苏州高泓利康生物科技有限公司
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