Five hinge regions and their chimeric antigen receptors and immune cells

[0064] 1. Entrust Shanghai Sangong Co., Ltd. to synthesize the corresponding target gene according to the nucleic acid sequence, where the target gene includes 4-1BB V (1-2-3-4-5), 4-1BB V (2-3-4-5) , 4-1BB V(3-4-5), 4-1BB V(4-5), 4-1BB V(5), No-H, CD8a-H, CD28-H and IgG4-H, where CD8a- The structural formula of H is intended as Figure 7 As shown, the nucleotide sequence of CD8a-H is shown in SEQ ID NO.18, and the amino acid sequence encoded by CD8a-H is shown in SEQ ID NO.19; the structural formula of CD28-H is intended to be as shown in Figure 8 As shown, the nucleotide sequence of CD28-H is shown in SEQ ID NO.20, the amino acid sequence encoded by CD28-H is shown in SEQ ID NO.21; the structural schematic diagram of IgG4-H is shown in Figure 9 As shown, the nucleotide sequence of IgG4-H is shown in SEQ ID NO.22, and the amino acid sequence encoded by IgG4-H is shown in SEQ ID NO.23. The target gene was connected to the vector pGEM4Z / GFP / A64 by means of gene cloning. We ...