A method for inoculating and propagating Puccinia maize

[0038] The inoculation propagation method (1) of embodiment 1 corn multi-heap Puccinia

[0039] The present embodiment provides a method for the inoculation and propagation of Puccinia maize multiheap, specifically as follows:

[0040] (1) Seedlings: select plump corn seeds, soak the seeds with 0.1% hydrogen peroxide at room temperature for 24h-48h, pour off the liquid or take out the seeds, cover the seeds with wet paper towels or wet cloth, and germinate in a ventilated place; The front end of the corn seed grows 1-2mm radicle before sowing. When sowing, the radicle faces upward and towards the center of the planting container. After cultivation, healthy corn seedlings at the three-leaf one-heart seedling stage are obtained;

[0041] (2) Spore elution on sick leaves and preparation of spore suspension: Spray 0.05% Tween-20 solution on sick leaves, about 2mL / sick leaves, cut sick leaves into 2-3cm 2 Place the small pieces in a large centrifuge tube, add 0.05% Tween-20 solut...

[0046] The inoculation propagation method (2) of embodiment 2 corn multi-heap Puccinia

[0047] This embodiment provides a method for inoculating and propagating Puccinia maize. The only difference between it and Embodiment 1 is that in step (5), steps (3) to (4) are repeated once.

[0048] Utilize the method of the present embodiment, after spraying the spore suspension, the spore heap on the blade is evenly distributed (such as figure 2 Shown); The harvested spores can obtain 3 pots of corn seedlings with good morbidity, and each pot harvests 30ng of spores, and obtains 100ng of spores altogether.